Evolutionary Analysis of Calcium-Dependent Protein Kinase in Five Asteraceae Species-猫眼课题宝

DOI：

10.3390/plants9010032

发表时间：

2019-12

期刊：

Plants

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

Liping Zhu;Bowen Zheng;Wangyang Song;Hongbin Li;Xiang Jin

钙依赖性蛋白激酶（CPK）是植物中一个重要的钙信号转导基因家族。在我们以前的工作中，我们报道了橡胶树CPKs在天然橡胶生物合成中的重要作用。然而，该CPK基因家族在其他橡胶生产植物中尚未被研究。在这里，我们报告的CPK在五个代表菊科物种，包括三个橡胶生产和两个非橡胶物种。从蒲公英、莴苣、向日葵、菊花和菜蓟中分别鉴定出34、34、40、34和30个CPKs。所有CPK被分为四个单独的组（组I至IV）。此外，还鉴定了10个TkCPKs、11个LsCPKs、20个HaCPKs、13个CnCPKs和7个CcCPKs重复的旁系同源物。进一步的进化分析表明，与其他亚科相比，类群III在菊科植物中，特别是在橡胶生产植物中得到了扩展。而菊科植物的CPK则有扩张的趋势，钙结合能力较低。本研究系统研究了菊科5种代表性植物CPKs的进化，结果表明，CPKs的亚家族特异性扩增可能与天然橡胶生产有关。

Calcium-dependent protein kinase (CPK) is crucial in Ca2+ signal transduction, and is a large gene family in plants. In our previous work, we reported Hevea brasiliensis CPKs were important for natural rubber biosynthesis. However, this CPK gene family in other rubber producing plants has not been investigated. Here, we report the CPKs in five representative Asteraceae species, including three rubber-producing and two non-rubber species. A total of 34, 34, 40, 34 and 30 CPKs were identified from Taraxacum koksaghyz, Lactuca sativa, Helianthus annuus, Chrysanthemum nankingense and Cynara cardunculus, respectively. All CPKs were classified into four individual groups (group I to IV). In addition, 10 TkCPKs, 11 LsCPKs, 20 HaCPKs, 13 CnCPKs and 7 CcCPKs duplicated paralogs were identified. Further evolutionary analysis showed that, compared to other subfamilies, the group III had been expanded in the Asteraceae species, especially in the rubber-producing species. Meanwhile, the CPKs in group III from Asteraceae species tend to expand with low calcium binding capacity. This study provides a systematical evolutionary investigation of the CPKs in five representative Asteraceae species, suggesting that the sub-family specific expansion of CPKs might be related to natural rubber producing.

Evolutionary Analysis of Calcium-Dependent Protein Kinase in Five Asteraceae Species

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