Beyond N and P: The impact of Ni on crude oil biodegradation
Beyond N and P: The impact of Ni on crude oil biodegradation
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DOI：
10.1016/j.chemosphere.2019.124545
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发表时间：
2019-12-01
期刊：
影响因子：
8.8
通讯作者：
Gray, Neil D.
中科院分区：
文献类型：
--
作者：
Mejeha, Obioma K.;Head, Ian M.;Gray, Neil D.
N 和 P 是被认为对刺激原油降解最重要的关键限制营养素，但其他微量营养素也可能很重要。设置实验土壤微观世界来研究镍修正背景下的原油降解。改性镍如 NiO、NiCl2 或卟啉复合物可以抑制、无效或增强石油污染土壤中的需氧碳氢化合物降解。相对于纯油对照，低水平 Ni-Porph (12 mg/kg) 的生物降解显着增强（95% 置信度）（70%）；而 NiO（200 和 350 mg/kg）显着抑制（36% 和 87%）生物降解，这与氧化物颗粒诱导的活性氧应激一致。微生物群落组成也受到镍的显着影响。在16S rRNA序列文库中，富集的碳氢化合物降解属红球菌被诺卡氏菌部分取代。存在低水平 NiO（12 和 50 mg/kg）的情况下。相比之下，红球菌的最高相对和绝对丰度与在 Ni-Porphamend 土壤中观察到的最大石油降解率一致。对镍依赖性脲酶或可能是镍依赖性超氧化物歧化酶（在红球菌基因组中发现）的生长依赖性构成需求为刺激提供了机制解释。这些结果表明，除了氮和磷之外，生物刺激技术还应该考虑微量营养素，例如镍，默认认为典型土壤中的镍是充分供应和可利用的。皇冠版权所有 (C) 2019 由 Elsevier Ltd 出版。保留所有权利。
N and P are the key limiting nutrients considered most important for the stimulation of crude oil degradation but other trace nutrients may also be important. Experimental soil microcosms were setup to investigate crude oil degradation in the context of Ni amendments. Amended Nickel as NiO, NiCl2, or, a porphyrin complex either inhibited, had no effect, or, enhanced aerobic hydrocarbon degradation in an oil-contaminated soil. Biodegradation was significantly (95% confidence) enhanced (70%) with low levels of Ni-Porph (12 mg/kg) relative to an oil-only control; whereas, NiO (200 and 350 mg/kg) significantly inhibited (36 and 87%) biodegradation consistent with oxide particle induced reactive oxygen stress. Microbial community compositions were also significantly affected by Ni. In 16S rRNA sequence libraries, the enriched hydrocarbon degrading genus, Rhodococcus, was partially replaced by a Nocardia sp. in the presence of low levels of NiO (12 and 50 mg/kg). In contrast, the highest relative and absolute Rhodococcus abundances were coincident with the maximal rates of oil degradation observed in the Ni-Porphamended soils. Growth dependent constitutive requirements for Ni-dependent urease or perhaps Ni-dependent superoxide dismutase enzymes (found in Rhodococcus genomes) provided a mechanistic explanation for stimulation. These results suggest biostimulation technologies, in addition to N and P, should also consider trace nutrients such as Ni tacitly considered adequately supplied and available in a typical soil. Crown Copyright (C) 2019 Published by Elsevier Ltd. All rights reserved.