ANTIGENIC PROPERTIES OF BORRELIA-BURGDORFERI ISOLATED FROM IXODES-OVATUS AND IXODES-PERSULCATUS IN HOKKAIDO, JAPAN
ANTIGENIC PROPERTIES OF BORRELIA-BURGDORFERI ISOLATED FROM IXODES-OVATUS AND IXODES-PERSULCATUS IN HOKKAIDO, JAPAN
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DOI：
10.1128/jcm.29.8.1568-1573.1991
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发表时间：
1991-08-01
影响因子：
9.4
通讯作者：
SATO, N
中科院分区：
文献类型：
--
作者：
MASUZAWA, T;OKADA, Y;SATO, N
日本莱姆病病原菌伯氏疏螺旋体HP3和HO14是日本首次发现的伯氏疏螺旋体，是1989年从日本北海道的全沟硬体和卵形硬体中分离到的。用抗B31株和P/Bi株的单抗，用免疫印迹法比较了日本株与美国(B31、297)和欧洲分离株(IRS、P/Gau、P/Bi2、2/B45、3/B56)的抗原性。日本株与抗41 kDa抗原的单抗U40反应。抗OSP A的单抗E34a31与除HP3菌株外的所有供试菌株均呈阳性反应。此外，抗OSP A的单抗U31b能与美国株和欧洲株反应，但不能与日本株反应。使用抗OSP B的单抗时，单抗E34b仅与欧洲株反应，单抗U34b仅与美国株反应。然而，这两种病毒对两种日本毒株都没有反应。抗60 kDa抗原的单抗E60能与所有欧美株和HP3株反应，但不能与日本株HO14反应。这些结果表明，从两种硬蜱中分离的日本株的抗原性与美国和欧洲分离的株不同。提示日本毒株比欧美毒株更适合作为日本莱姆病血清学诊断的抗原源。
Spirochete strains HP3 and HO14, isolated from Ixodes persulcatus and I. ovatus in Hokkaido in 1989, were the first isolates of Borrelia burgdorferi, the etiological agent of Lyme disease, to be recognized in Japan. Antigenic properties of the Japanese strains were compared with those of the strains isolated in the United States (B31 and 297) and Europe (IRS, P/Gau, P/Bi, 2/B45, and 3/B56) by Western blotting (immunoblotting), by using monoclonal antibodies (MAbs) against strains B31 and P/Bi. The Japanese strains reacted with MAb U40 against the 41-kDa antigen. MAb E34a31 against Osp A reacted with all the strains tested except for strain HP3. Furthermore, MAb U31b against Osp A reacted with all the American and European strains but did not react with the Japanese strains. When MAbs against Osp B were used, MAb E34b reacted only with European strains and MAb U34b reacted only with the American strains. However, neither showed reactivity to two Japanese strains. MAb E60 against 60-kDa antigen reacted with all the U.S. and European strains and strain HP3 but did not react with Japanese strain HO14. These results indicate that the antigenicity of the Japanese strains isolated from two species of ixodid ticks is different from that of the strains isolated in the United States and Europe. It is suggested that the Japanese strains are much more suitable than the U.S. or European strains as the antigen source for the serodiagnosis of Lyme disease in Japan.