EPIDERMAL-DERMAL TISSUE INTERACTIONS BETWEEN MUTANT AND NORMAL EMBRYONIC BACK SKIN - SITE OF MUTANT-GENE ACTIVITY DETERMINING ABNORMAL FEATHERING IS IN EPIDERMIS
EPIDERMAL-DERMAL TISSUE INTERACTIONS BETWEEN MUTANT AND NORMAL EMBRYONIC BACK SKIN - SITE OF MUTANT-GENE ACTIVITY DETERMINING ABNORMAL FEATHERING IS IN EPIDERMIS
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DOI：
10.1002/jez.1402000206
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发表时间：
1977-01-01
影响因子：
--
通讯作者：
BROTMAN, HF
中科院分区：
文献类型：
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作者：
BROTMAN, HF
通过将正常鸡胚和无鳞鸡胚的表皮和真皮进行重组，并将重组体在绒毛尿囊膜上培养7天，确定了无鳞基因在异常羽毛发育中的活性部位。当与共同的真皮来源重组时，与无鳞的低线表皮相比，无鳞的高线增强了羽毛发育。对一个共同的反应组织，7天的正常后表皮，羽毛诱导能力之间没有显着差异，正常，无鳞高线和无鳞低线真皮。与异常羽化有关，这些组织相互作用揭示了无鳞基因的活性部位是表皮。一个模型的组织相互作用的发展中的正常和异常羽毛。根据该模型，无鳞突变和通过选择高或低羽毛数量而积累的基因的焦点是表皮，其效果是表皮对皮肤刺激的反应性被改变。随后，表皮控制真皮的形态发生组织。无鳞真皮被认为包含用于确定羽毛结构和图案的正常位置信息。
The site of the scaleless gene''s activity in the development of abnormal feathers was determined by reciprocally recombining epidermis and dermis between normal and scaleless chick embryos and culturing the recombinants for 7 days on the chorioallantoic membrane. When recombined with a common dermal source, feather development is enhanced by scaleless high line as compared to scaleless low line epidermis. Against a common responding tissue, 7-day normal back epidermis, significant differences were not found in feather inducing ability between normal, scaleless high line and scaleless low line dermis. In relation to abnormal feathering, these tissue interactions reveal that the site of the scaleless gene''s activity is the epidermis. A model of tissue interactions in the development of normal and abnormal feathers is presented. According to the model, the focus of the scaleless mutation and the genes accumulated by selection for high or low feather numbers is the epidermis, the effect being that the reactivity of the epidermis to dermal stimuli is altered. Subsequently, the epidermis controls the morphogenetic organization of the dermis. The scaleless dermis is presumed to contain normal positional information for the determination of feather structure and pattern.