The migrational patterns and developmental fates of glial precursors in the rat subventricular zone are temporally regulated.
The migrational patterns and developmental fates of glial precursors in the rat subventricular zone are temporally regulated.
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DOI：
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发表时间：
1993-11
期刊：
影响因子：
4.6
通讯作者：
S. Levison;C. Chuang;B. Abramson;J. Goldman
中科院分区：
文献类型：
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作者：
S. Levison;C. Chuang;B. Abramson;J. Goldman
出生后的胶质细胞在啮齿动物前脑进行了研究，通过感染与复制缺陷型逆转录病毒编码的报告酶，使这些germinal区细胞的迁移和命运，以跟踪在随后的几个星期的新生儿或幼年大鼠脑室下区细胞。无论是新生儿还是青少年室管膜下区细胞迁移基本上沿着喙尾轴。新生室管膜下区细胞向背侧和外侧迁移进入大脑半球灰质和白色物质，成为星形胶质细胞和少突胶质细胞。幼年室管膜下区细胞迁移到皮质下白色物质的更内侧区域，有时出现在对侧半球的白色物质中，但很少迁移到新皮质中。幼年室管膜下区细胞几乎完全分化为少突胶质细胞。因此，室管膜下区细胞的迁移模式和发育命运在生命的前2周发生变化。当新生儿或青少年室管膜下区细胞在体内标记，然后取出和培养，一些产生同质克隆，包含星形胶质细胞与“1型”表型或少突胶质细胞，但有些产生异质克隆，包含两种胶质细胞类型。这些结果为星形胶质细胞和少突胶质细胞的共同祖细胞提供了额外的证据，并强烈表明，在出生后发育过程中，时间和空间调节的环境信号控制胶质祖细胞的命运。
Postnatal gliogenesis in the rodent forebrain was studied by infecting subventricular zone cells of either neonates or juvenile rats with replication-deficient retroviruses that encode reporter enzymes, enabling the migration and fate of these germinal zone cells to be traced over the ensuing several weeks. Neither neonatal nor juvenile subventricular zone cells migrated substantially along the rostral-caudal axis. Neonatal subventricular zone cells migrated dorsally and laterally into hemispheric gray and white matter and became both astrocytes and oligodendrocytes. Juvenile subventricular zone cells migrated into more medial areas of the subcortical white matter and on occasion appeared in the white matter of the contralateral hemisphere, but rarely migrated into the neocortex. Juvenile subventricular zone cells almost exclusively differentiated into oligodendrocytes. Thus, the migratory patterns and the developmental fates of subventricular zone cells change during the first 2 weeks of life. When either neonatal or juvenile subventricular zone cells were labeled in vivo and then removed and cultured, some generated homogeneous clones that contained either astrocytes with a 'type 1' phenotype or oligodendrocytes, but some generated heterogeneous clones that contained both glial types. These results provide additional evidence for a common progenitor for astrocytes and oligodendrocytes and strongly suggest that temporally and spatially regulated environmental signals control the destiny of glial progenitors during postnatal development.