High temperature crystallization of lysozyme: An example of phase transition
High temperature crystallization of lysozyme: An example of phase transition
复制标题
溶菌酶的高温结晶：相变的一个例子
DOI：
10.1016/0014-5793(72)80273-4
复制
发表时间：
1972
期刊：
影响因子：
3.5
通讯作者：
J. Berthou
中科院分区：
文献类型：
--
作者：
P. Jollés;J. Berthou
The crystallization of proteins is unfortunately very often almost empirical. If we could master external factors which are the easiest to handle, then perhaps more general methods could be established. From this point of view this report is an attempt to show the importance of constant temperatures (until 5.5’in this Note) for crystallization of enzymes. Experiments achieved in this sense with hen egg-white lysozyme (EC 3.2. 1.17) allowed besides to characterize a phase transition.The great variety of crystalline forms obtained from a given enzyme is a well known phenomenon. It depends mainly on internaZ factors: a) different representatives of an enzyme familycrystallize in quite different systems: hen [1], duck [2] and human [3] lysozyme crystals for example, are all different; b) when only one representative is considered (hen lysozyme) several crystalline forms have been described depending first on the ion bound to the enzyme: Cl; Br; NO;, SOi-[4] etc.; c) furthermore if we consider one form of hen lysozyme for example hydrochloride, we can obtain various types of crystals. Their occurrence depends on factors such as the protein concentration, the buffer, the pH, the precipitating salt, the presence of small amounts of heavy atoms, ions, dyes and traces of other materials [S, 61.
