The effect of cell population density on the developmental fate of reaggregating mouse limb bud mesenchyme.
The effect of cell population density on the developmental fate of reaggregating mouse limb bud mesenchyme.
复制标题
细胞群密度对重新聚集小鼠肢芽间充质发育命运的影响。
DOI：
10.1016/0012-1606(66)90048-0
复制
发表时间：
1966
影响因子：
2.7
通讯作者：
R. Umansky
中科院分区：
文献类型：
--
作者：
R. Umansky
Eleven-day embryonic C3H mouse limb buds were dissociated and the cells of the mesenchyme were permitted to reaggregate at controlled population densities on cellulose ester filter membranes of uniform surface area (pore size 0.45 μ). The results suggested that a large proportion of cells were capable of aggregating both as compact clusters and as monolayers, the relative proportions of each depending on initial cell population density on the membrane.Population-dependent aggregative patterns arising early in culture determined the nature of subsequent histodifferentiation and morphogenesis. Cartilage developed in cell clusters which, having undergone fusion, were able to retain compact cores. Myogenesis occurred on the surface of monolayer sheets.It is suggested that at the embryonic stage just prior to skeletal condensation in the limb bud, the great bulk of mesenchymal cells may be intrinsically capable of differentiating into more than one connective tissue type. Notwithstanding any covert differentiation that may have taken place, their fate is determined by the type of cellular associations establishedin vitro.
