Dynamic response of model lipid membranes to ultrasonic radiation force.
Dynamic response of model lipid membranes to ultrasonic radiation force.
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DOI：
10.1371/journal.pone.0077115
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Maduke MC
中科院分区：
文献类型：
--
作者：
Prieto ML;Ömer O;Khuri-Yakub BT;Maduke MC
低强度超声可以调节体内和体外神经元的动作电位放电。有人认为这种作用是由超声与神经细胞膜的机械相互作用介导的。我们研究了这些相互作用是否可以在合成脂质双分子层系统中进行进一步的研究。我们用电生理和激光多普勒振动仪测量了无蛋白模型膜对低强度超声的响应。我们发现超声波辐射力引起脂质膜的振荡和位移，导致膜面积和电容的变化很小（<1%）。在电压箝位下，电容的变化表现为电容电流，其时间过程呈指数衰减的正弦曲线。超声辐射力导致压力阶跃变化，破坏了双层张力和静水压力之间的平衡，膜振荡可以被建模为流体动力响应。我们还研究了作用在双分子层上的辐射力的来源。部分辐射力来自双分子层上方溶液/空气界面的超声波反射（这是我们实验配置特有的效应），但部分似乎反映了超声波与双分子层的直接相互作用，这与双分子层的声流或声散射有关。基于这些结果，我们认为合成脂质双分子层可以用于研究超声对细胞膜和膜蛋白的影响。
Low-intensity ultrasound can modulate action potential firing in neurons in vitro and in vivo. It has been suggested that this effect is mediated by mechanical interactions of ultrasound with neural cell membranes. We investigated whether these proposed interactions could be reproduced for further study in a synthetic lipid bilayer system. We measured the response of protein-free model membranes to low-intensity ultrasound using electrophysiology and laser Doppler vibrometry. We find that ultrasonic radiation force causes oscillation and displacement of lipid membranes, resulting in small (<1%) changes in membrane area and capacitance. Under voltage-clamp, the changes in capacitance manifest as capacitive currents with an exponentially decaying sinusoidal time course. The membrane oscillation can be modeled as a fluid dynamic response to a step change in pressure caused by ultrasonic radiation force, which disrupts the balance of forces between bilayer tension and hydrostatic pressure. We also investigated the origin of the radiation force acting on the bilayer. Part of the radiation force results from the reflection of the ultrasound from the solution/air interface above the bilayer (an effect that is specific to our experimental configuration) but part appears to reflect a direct interaction of ultrasound with the bilayer, related to either acoustic streaming or scattering of sound by the bilayer. Based on these results, we conclude that synthetic lipid bilayers can be used to study the effects of ultrasound on cell membranes and membrane proteins.
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影响因子：
3.4
作者：
Ismailov, II;Berdiev, BK;Benos, DJ
通讯作者：
Benos, DJ
影响因子：
3.4
作者：
CRAWFORD, GE;EARNSHAW, JC
通讯作者：
EARNSHAW, JC
影响因子：
2.3
作者：
Clement, GT;White, PJ;Hynynen, K
通讯作者：
Hynynen, K
DOI：
10.1109/tuffc.2010.1644
发表时间：
2010-09
期刊：
IEEE transactions on ultrasonics, ferroelectrics, and frequency control
影响因子：
--
作者：
McDannold N;Park EJ;Mei CS;Zadicario E;Jolesz F
通讯作者：
Jolesz F
影响因子：
5.3
作者：
Menz, Michael D.;Oralkan, Omer;Baccus, Stephen A.
通讯作者：
Baccus, Stephen A.