Involvement of transcription factors TCF-1 and GATA-3 in the initiation of the earliest step of T cell development in the thymus.
Involvement of transcription factors TCF-1 and GATA-3 in the initiation of the earliest step of T cell development in the thymus.
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DOI：
10.1084/jem.184.3.1137
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发表时间：
1996-09-01
期刊：
影响因子：
--
通讯作者：
Katsura Y
中科院分区：
文献类型：
--
作者：
Hattori N;Kawamoto H;Fujimoto S;Kuno K;Katsura Y
对含有针对各种表面标记物和转录因子的抗体的小鼠胎儿胸腺 (FT) 细胞进行流式细胞术和免疫细胞化学分析表明，TCF-1 和 GATA-3 蛋白的合成在最不成熟的 FT 细胞群的一部分中同时开始，这些细胞具有 CD4-CD8-CD44+CD25- 表型。在胎儿肝脏 (FL) 中未发现产生 TCF-1 的细胞。在CD44+CD25-FT细胞中，TCF-1的产生紧随CD3ε的细胞内表达。还发现，添加 TCF-1 或 GATA-3 的反义寡核苷酸会严重抑制 FT 器官培养系统中 FL（而非 FT）祖细胞的 T 细胞发育。这些结果强烈表明 TCF-1 和 GATA-3 在胸腺 T 细胞发育的最早步骤的启动中发挥着重要作用。
Flow cytometric and immunocytochemical analyses of murine fetal thymus (FT) cells with antibodies to various surface markers and transcription factors reveal that the synthesis of TCF-1 and GATA-3 protein begins simultaneously in a fraction of the most immature population of FT cells, which have the phenotype of CD4-CD8-CD44+CD25-. No TCF-1- producing cells is found in the fetal liver (FL). In CD44+CD25- FT cells, the production of TCF-1 is immediately followed by intracellular expression of CD3 epsilon. It is also found that the T cell development from FL, but not FT, progenitors in the FT organ culture system is severely inhibited by the addition of antisense oligonucleotides for either TCF-1 or GATA-3. These results strongly suggest that TCF-1 and GATA-3 play essential roles in the initiation of the earliest steps of T cell development in the thymus.