Urothelium patterns bladder smooth muscle location
Urothelium patterns bladder smooth muscle location
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DOI：
10.1203/pdr.0b013e318180e4c9
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发表时间：
2008-10-01
影响因子：
3.6
通讯作者：
Baskin, Laurence
中科院分区：
文献类型：
--
作者：
Cao, Mei;Liu, Benchun;Baskin, Laurence
在胚胎膀胱中，平滑肌分化是由位于浆膜附近的外周未分化间充质中的中央尿路上皮诱导的。我们假设，tinder 发出适当的信号，整个未分化的膀胱间充质能够分化平滑肌，并且尿路上皮模式纤维肌肉发育。野生型和绿色荧光蛋白小鼠的胚胎膀胱在平滑肌分化之前被分成尿路上皮和间质成分（E12.5-E13）。尿路上皮层绿色荧光蛋白在原位、异位和异位与间充质（野生型）重组并嫁接。在所有情况下，无论尿路上皮处于原位还是异型位置，都在上皮附近观察到平滑肌抑制区。单独移植的膀胱间质和膀胱上皮不生长。总之，膀胱间质的全层能够进行平滑肌分化，依赖于尿路上皮的位置。这些实验支持这样的假设，即尿路上皮分泌一种扩散因子，高浓度时抑制平滑肌，低浓度时诱导平滑肌，从而在纤维肌性膀胱壁的整个厚度上形成间充质细胞分化模式。
Smooth muscle differentiation is induced ill the embryonic bladder by the centrally located urothelium in the undifferentiated mesenchyme in the periphery adjacent to the serosa. We hypothesize that tinder the appropriate signal the entire undifferentiated bladder mesenchyme is capable of smooth muscle differentiation and that the urothelium patterns fibromuscular development. Embryonic bladders of wild-type and Green Fluorescent Protein mice were separated into urothelial and mesenchymal components before smooth muscle differentiation (E12.5-E13). The urothelial layer,green fluorescent protein was recombined and grafted with the mesenchyme (wild-type) in an orthotopic position, heterotopic position and ectopic position. In all cases, a zone of smooth muscle inhibition was observed adjacent to the epithelium whether the urothelium was ill ail orthotopic or heterotypic position. Bladder mesenchyme and bladder epithelium grafted alone did not grow. In conclusion, the full thickness of bladder mesenchyme is capable of smooth muscle differentiation dependent oil the location of urothelium. These experiments support the hypothesis that urothelium secretes a diffusible factor that Lit high concentrations inhibits smooth Muscle and at low concentrations induces smooth muscle, thus patterning mesenchymal cell differentiation across the full thickness of the fibromuscular bladder wall.