The peripheral blood fibrocyte is a potent antigen-presenting cell capable of priming naive T cells in situ
The peripheral blood fibrocyte is a potent antigen-presenting cell capable of priming naive T cells in situ
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DOI：
10.1073/pnas.94.12.6307
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发表时间：
1997-06-10
影响因子：
11.1
通讯作者：
Bucala, R
中科院分区：
文献类型：
--
作者：
Chesney, J;Bacher, M;Bucala, R
最近的研究发现了一种新的血细胞群，称为纤维细胞，具有独特的细胞表面表型（胶原（+）/CD 13（+）/CD 34（+）/CD 45（+）），迅速进入组织损伤部位，并合成结缔组织基质分子。我们通过流式细胞术发现纯化的人纤维细胞表达抗原呈递所需的每种已知表面组分，包括II类主要组织相容性复合物分子（HLA-DP、DQ和-DR），共刺激分子CD 80和CD 86，以及粘附分子CD 11 a、CD 54和CD 58，人纤维细胞诱导的抗原提呈细胞-当与特异性抗原一起培养时，依赖性T细胞增殖，并且这种增殖活性显著高于单核细胞诱导的增殖活性，并且几乎与小鼠成纤维细胞经纯化的树突状细胞诱导后，也可表达抗原呈递所需的表面成分，并在体外发挥有效的APC功能。小鼠纤维细胞在体外用HIV蛋白p24或gp 120脉冲并递送到皮肤损伤部位，发现其仅迁移到近端淋巴结并特异性地引发幼稚T细胞。这些数据表明，纤维细胞在抗原特异性免疫的启动中起着早期和重要的作用。
Recent studies have identified a novel population of blood-borne cells, termed fibrocytes, that have a distinct cell surface phenotype (collagen(+)/CD13(+)/CD34(+)/CD45(+)), rapidly enter sites of tissue injury, and synthesize connective tissue matrix molecules, We found by flow cytometry that purified human fibrocytes express each of the known surface components that are required for antigen presentation, including class II major histocompatability complex molecules (HLA-DP, DQ, and -DR), the costimulatory molecules CD80 and CD86, and the adhesion molecules CD11a, CD54, and CD58, Human fibrocytes induced antigen-presenting cell-dependent T cell proliferation when cultured with specific antigen and this proliferative activity was significantly higher than that induced by monocytes and nearly as high as that induced by purified dendritic cells, Mouse fibrocytes also were found to express the surface components required for antigen presentation and to function as potent APCs in vitro. Mouse fibrocytes pulsed in vitro with the HIV-proteins p24 or gp120 and delivered to a site of cutaneous injury mere found to migrate to proximal lymph nodes and to specifically prime naive T cells. These data suggest that fibrocytes play an early and important role in the initiation of antigen-specific immunity.