A plasmid isolated from phytopathogenic onion yellows phytoplasma and its heterogeneity in the pathogenic phytoplasma mutant.
A plasmid isolated from phytopathogenic onion yellows phytoplasma and its heterogeneity in the pathogenic phytoplasma mutant.
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从植物病原洋葱中分离的质粒使植原体变黄及其在病原植原体突变体中的异质性。
DOI：
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发表时间：
1998
影响因子：
3.5
通讯作者：
S. Namba
中科院分区：
文献类型：
--
作者：
T. Kuboyama;C. C. Huang;X. Lu;T. Sawayanagi;T. Kanazawa;T. Kagami;I. Matsuda;T. Tsuchizaki;S. Namba
从病原植物软体洋葱黄化植原体 (OY-W) 的染色体外 DNA 中克隆了 3.6-kbp DNA 片段。该片段的序列分析揭示了编码滚环复制（RCR）型质粒的复制（Rep）蛋白的开放阅读框（ORF）。该结果表明OY-W中存在使用RCR机制的质粒(pOYW1)。通过检测 RCR 机制专门产生的复制中间体的单链 DNA (ssDNA)，证实了这一假设。这是对该质粒的复制系统及其编码基因的鉴定的第一份报告。以包含 pOYW1 Rep 基因区域的 DNA 片段作为探针，采用 Southern 和 Northern (RNA) 印迹杂交来检查 OY-W 中发现的质粒与从 OY-W 中分离的致病突变体 (OY-M) 之间的异质性。从 OY-W 和 OY-M 感染植物中提取的 DNA 和 RNA 中均检测到多个条带，但条带模式不同。此外，OY-W 的质粒拷贝数比 OY-M 的质粒拷贝数高约 4.2 倍。这些结果表明OY-W和OY-M质粒之间存在结构异质性，并提示质粒编码基因与植原体致病性之间存在关系的可能性。
A 3.6-kbp DNA fragment was cloned from the extrachromosomal DNA of a pathogenic plant mollicute, onion yellows phytoplasma (OY-W). Sequence analysis of the fragment revealed an open reading frame (ORF) encoding the replication (Rep) protein of rolling-circle replication (RCR)-type plasmids. This result suggests the existence of a plasmid (pOYW1) in OY-W that uses the RCR mechanism. This assumption was confirmed by detecting the single-stranded DNA (ssDNA) of a replication intermediate that is specifically produced by the RCR mechanism. This is the first report on the identification of the replication system of this plasmid and the genes encoded in it. With a DNA fragment including the Rep gene region of pOYW1 used as a probe, Southern and Northern (RNA) blot hybridizations were employed to examine the heterogeneity between the plasmids found in OY-W and a pathogenic mutant (OY-M) isolated from OY-W. Multiple bands were detected in the DNA and RNA extracted from both OY-W and OY-M infected plants, although the banding patterns were different. Moreover, the copy number of plasmids from OY-W was about 4.2 times greater than that from OY-M. These results indicate constructive heterogeneity between OY-W and OY-M plasmids, and the possibility of a relationship between the plasmid-encoded genes and the pathogenicity of the phytoplasma was suggested.
对与 DNA 和 RNA 狭缝印迹杂交的地高辛标记 DNA 进行定量。
影响因子：
2.9
作者：
Krueger,SK;Williams,DE
通讯作者：
Williams,DE
丝状支原体亚种丝状支原体质粒pKMK1的核苷酸序列。
影响因子：
2.6
作者：
King,KW;Dybvig,K
通讯作者：
Dybvig,K