Kinetics of Borrelia burgdorferi dissemination and evolution of disease after intradermal inoculation of mice.
Kinetics of Borrelia burgdorferi dissemination and evolution of disease after intradermal inoculation of mice.
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发表时间：
1991-08
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通讯作者：
S. Barthold;D. Persing;Audrey L. Armstrong;Ruth A. Peeples
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作者：
S. Barthold;D. Persing;Audrey L. Armstrong;Ruth A. Peeples
在皮内接种4周龄C3 H小鼠后第1、2、3、4、7、10、15、21和30天检查伯氏疏螺旋体向选定靶器官的传播。通过培养（血液、脾脏、肾脏、耳穿孔）;外表面蛋白A（OSP A）DNA的聚合酶链反应（PCR）（耳穿孔）;组织学和螺旋体组织化学（脾脏、肾脏、皮肤、心脏、关节）;以及OSP A DNA原位杂交（关节、心脏）确定感染。大多数小鼠的血液或脾脏在第3天培养阳性，在第10天耳穿孔阳性。在第10天，对耳钻进行的聚合酶链反应也呈阳性。关节和肌腱的炎症在第4天至第7天开始，心脏在第7天至第10天开始，这与螺旋体在组织中的定殖相一致。螺旋体分布呈多灶性，好发于关节、心脏、动脉、神经、肌肉、皮肤和其他组织的胶原结缔组织。螺旋体的相对数量在第15天达到峰值，然后在第21天下降。γ-M免疫球蛋白（IgM）抗体可检测免疫印迹早在第4天，随后下降的反应性，IgG抗体可检测到的第7天，扩大反应性多种抗原通过第30天。
Borrelia burgdorferi dissemination to selected target organs was examined on days 1, 2, 3, 4, 7, 10, 15, 21, and 30 after intradermal inoculation of 4-week-old C3H mice. Infection was determined by culture (blood, spleen, kidney, ear punch); polymerase chain reaction (PCR) for outer surface protein A (OSP A) DNA (ear punch); histology and spirochete histochemistry (spleen, kidney, skin, heart, joints); and OSP A DNA in situ hybridization (joints, heart). Blood or spleen of most mice were culture positive by day 3 and ear punch by day 10. Polymerase chain reaction performed on ear punches was also positive by day 10. Inflammation of joints and tendons began on days 4 through 7 and heart on days 7 through 10, which coincided with colonization of tissues with spirochetes. Spirochetes were multifocal in distribution, with a predilection for collagenous connective tissue of joints, heart, arteries, nerves, muscle, skin, and other tissues. Relative numbers of spirochetes peaked at 15 days, then decreased by 21 days. Gamma M immunoglobulin (IgM) antibody was detectable on immunoblots as early as day 4, with subsequent declining reactivity, and IgG antibody was detectable by day 7, with expanding reactivity to multiple antigens through day 30.