Piezo-resistive method for tuberculosis detection using microcantilever biosensor
Piezo-resistive method for tuberculosis detection using microcantilever biosensor
复制标题
使用微悬臂梁生物传感器检测结核病的压阻法
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
W. Patil
中科院分区：
文献类型：
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作者：
S. Dhakane;W. Patil
微悬臂梁器件被应用于多种生物传感器中，用于检测感兴趣的生物分子。生物传感器的任务是识别目标分子的存在，并将结果提供给可测量的信号。与传统的生物仪器相比，微型生物传感器具有快速、可靠、性价比高等优点。微电子机械系统(MEMS)/纳米电子机械系统被用来设计这种生物传感器，它可能在几个生物学应用中被利用。生物传感器的传感机理因应用的不同而不同。本论文主要研究利用微悬臂梁进行生物分子检测。生物传感器采用压阻法检测目标生物分子。利用Coventorware软件对该Bio-MEMS器件进行了设计和仿真。这个生物传感器想要在一个非常可疑的病人身上识别出结核杆菌的存在。为了检测初发期结核病(ESAT-6)，需要将特异性抗体固定在微悬臂梁上。病人标本(由ESAT-6组成)一旦被放置在功能化的悬臂梁表面，结核抗原ESAT-6与抗体之间就会发生生化反应。抗原的吸附会增加悬臂梁上的作功质量，导致微悬臂梁的弯曲。这种偏转表明患者样本中存在结核病。
Microcantilever device is utilized in several biosensors for detection of bio-molecule of interest. Biosensors task is to identify presence of targeted molecule and supply result into a measurable signal. Comparing with conventional biological equipments, micro scale biosensors are very fast, reliable and price effective. Microelectromechanical systems (MEMS)/nanoelectromechanical systems are used for designing of such biosensors, which might be utilized in several biological applications. Sensing mechanism of biosensor is varied with the application. This paper is focused on detection of bio-molecule using microcantilever beam. Biosensor has shown here uses piezo-resistive method for detection of targeted bio-molecule. This Bio-MEMS device is designed and simulated using coventorware software. This biosensor wants to identify a presence of T.B. in a very suspected patient. For detection of primary stage of tuberculosis (ESAT-6), specific antibodies need to immobilize on top of microcantilever. Once patient sample (consist of ESAT-6) is placed on functionalized cantilever surface, biochemical reaction happen between tuberculosis antigens ESAT-6 and antibodies. Adsorption of antigens will increase mass working on cantilever and results in bending of microcantilever. This deflection shows the presence of tuberculosis within the patient sample.
影响因子：
10.7
作者：
G. Litman;J. Rast;M. Shamblott;R. Haire;M. Hulst;W. Roess;R. Litman;K. R. Hinds-Frey;Anna Zilch;Chris T. Amemiya
通讯作者：
G. Litman;J. Rast;M. Shamblott;R. Haire;M. Hulst;W. Roess;R. Litman;K. R. Hinds-Frey;Anna Zilch;Chris T. Amemiya
影响因子：
7.4
作者：
Nelson, BP;Grimsrud, TE;Corn, RM
通讯作者：
Corn, RM