RNA sequencing reveals pronounced changes in the noncoding transcriptome of aging synaptosomes
RNA sequencing reveals pronounced changes in the noncoding transcriptome of aging synaptosomes
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DOI：
10.1016/j.neurobiolaging.2017.04.005
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发表时间：
2017-08-01
影响因子：
4.2
通讯作者：
Janitz, Michael
中科院分区：
文献类型：
--
作者：
Chen, Bei Jun;Ueberham, Uwe;Janitz, Michael
Normal aging is associated with impairments in cognitive functions. These alterations are caused by diminutive changes in the biology of synapses, and ineffective neurotransmission, rather than loss of neurons. Hitherto, only a few studies, exploring molecular mechanisms of healthy brain aging in higher vertebrates, utilized synaptosomal fractions to survey local changes in aging-related transcriptome dynamics. Here we present, for the first time, a comparative analysis of the synaptosomes transcriptome in the aging mouse brain using RNA sequencing. Our results show changes in the expression of genes contributing to biological pathways related to neurite guidance, synaptosomal physiology, and RNA splicing. More intriguingly, we also discovered alterations in the expression of thousands of novel, un-annotated lincRNAs during aging. Further, detailed characterization of the cleavage and polyadenylation factor I subunit 1 (Clp1) mRNA and protein expression indicates its increased expression in neuronal processes of hippocampal stratum radiatum in aging mice. Together, our study uncovers a new layer of transcriptional regulation which is targeted by aging within the local environment of interconnecting neuronal cells. (C) 2017 Elsevier Inc. All rights reserved.
