Guidelines for the next 10 years of proteomics
Guidelines for the next 10 years of proteomics
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DOI：
10.1002/pmic.200500856
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发表时间：
2006-01-01
期刊：
影响因子：
3.4
通讯作者：
Dunn, MJ
中科院分区：
文献类型：
--
作者：
Wilkins, MR;Appel, RD;Dunn, MJ
在过去的十年里，蛋白质组学领域迅速扩张。一系列令人兴奋的新技术已经被开发出来，并被热情地应用于各种生物学问题。然而，蛋白质组数据生成和分析所需的严格程度似乎被低估了。因此，很可能会有许多已发表的调查结果质量有问题，需要进一步确认和/或确认。这份手稿概述了蛋白质组学研究中的一些关键问题，包括与实验设计、差异展示和生物标记物发现相关的问题；蛋白质鉴定和分析的不完整性。为了建立一个标准，以反映目前对该领域可出版手稿的必要和可取特征的思考，然后描述了蛋白质组学研究的最小指南集。这些指南将作为一套标准，蛋白质组学的编辑将用来评估未来向该杂志提交的论文。
In the last ten years, the field of proteomics has expanded at a rapid rate. A range of exciting new technology has been developed and enthusiastically applied to an enormous variety of biological questions. However, the degree of stringency required in proteomic data generation and analysis appears to have been underestimated. As a result, there are likely to be numerous published findings that are of questionable quality, requiring further confirmation and/or validation. This manuscript outlines a number of key issues in proteomic research, including those associated with experimental design, differential display and biomarker discovery; protein identification and analytical incompleteness. In an effort to set a standard that reflects current thinking on the necessary and desirable characteristics of publishable manuscripts in the field, a minimal set of guidelines for proteornics research is then described. These guidelines will serve as a set of criteria which editors of PROTEOMICS will use for assessment of future submissions to the journal.