Expression and Functional Characterization of the Adhesion Molecule Spermatogenic Immunoglobulin Superfamily in the Mouse Testis1
Expression and Functional Characterization of the Adhesion Molecule Spermatogenic Immunoglobulin Superfamily in the Mouse Testis1
复制标题
小鼠睾丸中粘附分子生精免疫球蛋白超家族的表达和功能表征
作者：
T. Wakayama;H. Koami;Hiroyuki Ariga;Daisuke Kobayashi;Y. Sai;A. Tsuji;Miyuki Yamamoto;S. Iseki
摘要生精免疫球蛋白超家族（SgIGSF）是小鼠生精小管生精细胞表达的免疫球蛋白超家族成员。我们制备了抗SgIGSF的特异性多克隆抗体。使用该抗体对出生后发育小鼠的睾丸进行Western印迹分析，显示出80-130 kDa的多个免疫阳性条带，其数量和大小随出生后年龄增加。酶促N-糖酵解导致这些条带的大小减少到70 kDa，表明SgIGSF是一种糖蛋白，其糖基化模式和程度受发育调节。成年睾丸的免疫组化分析表明，SgIGSF是存在于生精细胞中的精子发生的早期步骤，并从中间精原细胞通过偶线期精母细胞的量增加，但减少的步骤，从粗线期早期精母细胞通过圆形精子细胞。减数分裂后，SgIGSF再次出现在第7步精子细胞中，并存在于伸长精子细胞中，直到精子形成。免疫反应主要位于细胞膜上。与成人睾丸中的发现一致，对发育中的睾丸的分析显示，SgIGSF在早期和晚期的生精细胞中分别表达。支持细胞没有表达SgIGSF，而从睾丸裂解物中免疫沉淀和在COS-7细胞中产生的SgIGSF显示结合到原代培养的支持细胞表面。这些结果表明，生精细胞表面的SgIGSF以异嗜性方式与支持细胞上的某些膜分子结合，从而可能在精子发生中发挥多种作用。
Abstract Spermatogenic immunoglobulin superfamily (SgIGSF) is a mouse protein belonging to the immunoglobulin superfamily expressed in the spermatogenic cells of seminiferous tubules. We produced a specific polyclonal antibody against SgIGSF. Western blot analysis of the testes from postnatal developing mice using this antibody demonstrated multiple immunopositive bands of 80–130 kDa, which increased in number and size with the postnatal age. Enzymatic N-glycolysis caused reduction in the size of these bands to 70 kDa, indicating that SgIGSF is a glycoprotein and its glycosylation pattern and extent are developmentally regulated. Immunohistochemical analysis of the adult testis demonstrated that SgIGSF was present in the spermatogenic cells in the earlier steps of spermatogenesis and increased in amount from intermediate spermatogonia through zygotene spermatocytes but was diminished in the steps from early pachytene spermatocytes through round spermatids. After meiosis, SgIGSF reappeared in step 7 spermatids and was present in the elongating spermatids until spermiation. The immunoreactivity was localized primarily on the cell membrane. Consistent with the findings in adult testes, the analysis of the developing testes revealed that SgIGSF was expressed separately in the spermatogenic cells in earlier and later phases. Sertoli cells had no expression of SgIGSF, whereas both SgIGSF immunoprecipitated from the testis lysate and produced in COS-7 cells was shown to bind to the surface of Sertoli cells in primary culture. These results suggested that SgIGSF on the surface of spermatogenic cells binds to some membrane molecules on Sertoli cells in a heterophilic manner and thereby may play diverse roles in the spermatogenesis.
哺乳动物精子后尾质膜结构域的生物发生：精子发生过程中后尾结构域特异性跨膜蛋白CE9的靶向和横向重新分布。
DOI：
10.1006/dbio.1995.1162
发表时间：
1995
期刊：
Developmental biology.
影响因子：
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作者：
Cesario,MM;Ensrud,K;Hamilton,DW;Bartles,JR
通讯作者：
Bartles,JR