RELEASE OF LIPOPOLYSACCHARIDE BY EDTA TREATMENT OF E COLI
RELEASE OF LIPOPOLYSACCHARIDE BY EDTA TREATMENT OF E COLI
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DOI：
10.1016/0006-291x(65)90191-9
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发表时间：
1965-01-01
影响因子：
3.1
通讯作者：
LEIVE, L
中科院分区：
文献类型：
--
作者：
LEIVE, L
用乙二胺四乙酸酯处理大肠杆菌，使其渗透性增加，也导致35%至50%的细胞壁脂多糖的快速释放。由于相同的条件下，其他几种细胞成分的损失可以忽略不计，因此脂多糖的释放可能是EDTA作用于细胞表面的直接结果。这种处理方法可能是分离化学纯脂多糖的有用工具。
The treatment of E., coli with ethylenediaminetetraacetate which causes an increase in permeability also results in the rapid release of 35 to 50% of the cell wall lipopolysaccharide. Since the same conditions produce negligible loss of several other cell components, it is possible that the release of lipopolysaccharide is a direct result of EDTA action on the cell surface. This treatment may be a useful tool in the isolation of chemically pure lipopolysaccharide.