Antigen-specific and non-specific CD4+ T cell recruitment and proliferation during influenza infection
Antigen-specific and non-specific CD4+ T cell recruitment and proliferation during influenza infection
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DOI：
10.1016/j.virol.2005.06.023
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发表时间：
2005-09-30
期刊：
影响因子：
3.7
通讯作者：
Topham, DJ
中科院分区：
文献类型：
--
作者：
Chapman, TJ;Castrucci, MR;Topham, DJ
为了在流感感染期间在单细胞水平上跟踪表位特异性CD4(+) T细胞，将MHC ii类限制性OVA（323-339）表位设计到流感/AIWSN的神经氨酸酶柄中，产生替代病毒抗原。重组病毒流感A/WSN/OVA（II）复制良好，被正常清除，刺激野生型和DO11.10或OT-II TCR转基因OVA特异性CD4(+) T细胞。只有当OVA表位存在时，OVA特异性CD4 T细胞才会在感染期间增殖。然而，在存在或不存在OVA323-339表位的情况下，先前启动的（但不是幼稚的）转基因CD4(+) T细胞被招募到感染的肺部。这些数据表明，当启动时，CD4(+) T细胞可以在没有抗原的情况下运输到肺，但不增殖。这些结果也为研究CD4 T细胞在流感感染中的作用提供了一个有用的工具。(c) 2005爱思唯尔公司版权所有。
To track epitope-specific CD4(+) T cells at a single-cell level during influenza infection, the MHC class II-restricted OVA(323-339) epitope was engineered into the neuraminidase stalk of influenza/AIWSN, creating a surrogate viral antigen. The recombinant virus, influenza A/WSN/OVA(II), replicated well, was cleared normally, and stimulated both wild-type and DO11.10 or OT-II TCR transgenic OVA-specific CD4(+) T cells. OVA-specific CD4 T cells proliferated during infection only when the OVA epitope was present. However, previously primed (but not naive) transgenic CD4(+) T cells were recruited to the infected lung both in the presence and absence of the OVA323-339 epitope. These data show that, when primed, CD4(+) T cells may traffic to the lung in the absence of antigen, but do not proliferate. These results also document a useful tool for the study of CD4 T cells in influenza infection. (c) 2005 Elsevier Inc. All rights reserved.