ROLE OF CALCIUM IN INITIATION OF ACTIVITY OF SMOOTH MUSCLE
ROLE OF CALCIUM IN INITIATION OF ACTIVITY OF SMOOTH MUSCLE
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DOI：
10.1152/ajplegacy.1969.216.3.671
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发表时间：
1969-01-01
影响因子：
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通讯作者：
BOZLER, E
中科院分区：
文献类型：
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作者：
BOZLER, E
方法采用宽2 ~ 3mm的龟主动脉和蛙胃肌环和大鼠子宫纵条。将它们悬浮在10ml溶液中。张力被记录在测谎仪上，使用Grass FT03作为传感器。在主动脉的自发收缩过程中产生的张力通常为1 -2克。冷血动物用林格液，含（mM）： NaCl, 115；氯化钾2;CaC12, 1: MgSO4, 0.6；NaHCOs 4;NaH2POa, 0.5。在pH影响的实验中，仅使用磷酸盐缓冲液。含（mM）： NaCl, 140；氯化钾,2.5;NaHCOz 9;NaH2P04, 0.5，葡萄糖，5。氧气不断地在溶液中冒泡。以Geigy化工公司生产的乙二氧双（乙烯氨基）四乙酸（EGTA）为原料，加入0.1 M溶液，经KOH调节pH为7。在实验开始时，每隔约10分钟更换两次无ca溶液。药物的浓度以每毫升克为单位表示。主动脉和胃肌实验在室温（24-26℃）下进行，子宫肌实验在35℃下进行。
METHODSRings of the aorta of the turtle and of the stomach muscle of the frog, both 2-3 mm wide, and longitudinal strips of the rat’s uterus were used. They were suspended in 10 ml solution. Tension was recorded on a polygraph using a Grass FT03 as transducer. The tension produced during a spontaneous cont. raction of preparations of the aorta was usually l-2 g.Ringer solution for cold-blooded animals contained (in mM): NaCl, 115; KCl, 2; CaC12, 1: MgSO4, 0.6; NaHCOs, 4; NaH2POa, 0.5. In experiments on the effect of pH only phosphate buffers were used. Mam-malian Ringer solution contained (in mM): NaCl, 140; KCl, 2.5; NaHCOz, 9; NaH2P04, 0.5, glucose, 5. Oxygen bubbled through the solution continuously. Ethylenedioxybis (ethyleneamino) tetraaceticacid (EGTA), obtained from Geigy Chemical Corporation, was added as 0.1 M solution adjusted to pH 7 by KOH. Ca-free solutions were changed twice at the beginning of an experiment at intervals of about 10 min. The concentration of drugs is given as gram base per milliliter. The experiments on aorta and stomach muscle were carried out at room temperature(24-26 C), those on uterine muscle at 35 C.