Diversity of the expression profiles of late embryogenesis abundant (LEA) protein encoding genes in the anhydrobiotic midge Polypedilum vanderplanki
Diversity of the expression profiles of late embryogenesis abundant (LEA) protein encoding genes in the anhydrobiotic midge Polypedilum vanderplanki
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DOI：
10.1007/s00425-015-2284-6
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发表时间：
2015-08-01
期刊：
影响因子：
4.3
通讯作者：
Kikawada, Takahiro
中科院分区：
文献类型：
--
作者：
Hatanaka, Rie;Gusev, Oleg;Kikawada, Takahiro
主要结论在脱水生活的范氏多足蠓中，莱亚家族蛋白在幼虫的脱水和复水过程中可能发挥着不同的时间和空间作用，能够耐受几乎完全脱水的范氏多足蠓幼虫积累了胚胎发育后期丰富（莱亚）蛋白，以响应干燥。利用完整的基因组数据的吸浆虫，我们已经确定了27 PvLea 1样基因的基础上的相似性，以前的特点PvLea 1基因属于组3莱亚蛋白。通常，第3组莱亚蛋白的特征在于11-mer基序的几个重复。然而，一些PvLea基因在其序列中缺乏典型基序。我们对所有27个PvLea基因的生化和生物物理特性以及保守基序进行了详细的表征。氨基酸序列的基序分析表明，所有27个Pv莱亚蛋白都具有两种基序中的至少一种（基序1：GAKDTTKEKLGE AKDATAEKLG或基序2：KD ILExAKDKLxD AKDAVKEKL），表明存在至少两个重复的11-mer莱亚基序。大部分PvLEA蛋白定位于细胞质中。我们还进行了定量实时PCR的所有27个PvLea基因的详细过程中的干燥和复水。这些基因的表达在脱水的开始，后期的干燥过程和复水过程中上调。这些数据表明，每个莱亚蛋白可能发挥不同的时间和空间的作用，在整个过程中的干燥和复水的幼虫。
Main conclusion In the anhydrobiotic midge Polypedilum vanderplanki , LEA family proteins are likely to play distinct temporal and spatial roles in the larvae throughout the process of desiccation and rehydration.The larvae of the anhydrobiotic midge, P. vanderplanki, which can tolerate almost complete desiccation, accumulate late embryogenesis abundant (LEA) proteins in response to drying. Using complete genome data of the midge, we have identified 27 PvLea1-like genes based on the similarity to previously characterized PvLea1 gene belonging to group 3 LEA proteins. Generally, group 3 LEA proteins are characterized by several repetitions of an 11-mer motif. However, some PvLea genes lack the canonical motif in their sequences. We performed the detailed characterization of all 27 PvLea genes in terms of biochemical and biophysical properties and conserved motifs. The motif analysis among their amino acid sequences revealed that all 27 PvLEA proteins have at least one of two types of motifs (motif 1: G AKDTTKEKLGE AKDATAEKLG or motif 2: KD ILExAKDKLxD AKDAVKEKL), indicating the presence of at least two repeated 11-mer LEA motifs. Most of PvLEA proteins were localized to the cytosol. We also performed quantitative real-time PCR of all 27 PvLea genes in detail during the process of desiccation and rehydration. The expression of these genes was upregulated at the beginning of dehydration, the latter phase of the desiccation process and on rehydration process. These data suggested that each LEA protein is likely to play distinct temporal and spatial roles in the larvae throughout the process of desiccation and rehydration.