CYTO-TOXIC EFFECTS OF METHYLNITROSOUREA ON DEVELOPING BRAIN
CYTO-TOXIC EFFECTS OF METHYLNITROSOUREA ON DEVELOPING BRAIN
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DOI：
10.1007/bf00963920
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发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
OMORI, Y
中科院分区：
文献类型：
--
作者：
FUJIMORI, K;SUNOUCHI, M;OMORI, Y
甲基亚硝基脲（MNU）对小脑和大脑DNA、RNA、蛋白质、溶酶体酶（酸性DNA酶、RNA酶、磷酸酶和β-半胱氨酸酶）的作用葡萄糖醛酸酶）和2“，3”-环核苷酸3“-磷酸水解酶（2”，3“-CN酶）的活性进行了研究。S.C.注射MNU 0.625mmol/kg可抑制小脑重量和DNA、RNA及蛋白质含量的增加，但对大脑重量和体重无明显影响。蛋白质和RNA与DNA的比率在小脑中被MNU显著升高，但在大脑中没有。酸性DNA酶和酸性RNA酶的活动的MNU治疗的大鼠显着升高超出了这些活动的增加，在小脑中的控制，但在这些活动的MNU没有观察到变化。MNU预处理后，小脑酸性磷酸酶活性略有升高，但大脑中没有。β-的葡萄糖醛酸酶和2“，3”-CN酶活性在小脑或大脑中没有变化。在发育中的大脑，特别是在小脑有丝分裂阶段，MNU引起细胞损伤和抑制细胞有丝分裂。
The effect of methylnitrosourea (MNU) on cerebellar and cerebral DNA, RNA, protein, lysosomal enzymes (acid DNase, RNase, phosphatase and .beta.-glucuronidase) and 2'',3''-cyclic nucleotide 3''-phosphohydrolase (2'',3''-CNPase) activities was studied in rats from birth through 12 days of age. S.c. injection of MNU 0.625 mmol/kg caused a suppression of increase in weights and content of DNA, RNA and protein of cerebellum, but no changes in those of the cerebrum or in body weight. Ratios of protein and RNA to DNA were substantially elevated by MNU in the cerebellum but not in the cerebrum. Acid DNase and acid RNase activities of MNU-treated rats were significantly elevated beyond the increase of these activities in controls in the cerebellum, but no change in these activities by MNU was observed in the cerebrum. A slight elevation in acid phosphatase activity was observed in the cerebellum but not in the cerebrum after MNU pretreatment. .beta.-Glucuronidase and 2'',3''-CNPase activities were not changed in the cerebellum or cerebrum. In the developing brain, especially in the cerebellum at the mitotic stage, MNU caused cell damage and inhibited cell mitosis.