Presence of the blaZ beta-lactamase gene in isolates of Staphylococcus aureus that appear penicillin susceptible by conventional phenotypic methods
Presence of the blaZ beta-lactamase gene in isolates of Staphylococcus aureus that appear penicillin susceptible by conventional phenotypic methods
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DOI：
10.1016/j.diagmicrobio.2012.07.013
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发表时间：
2012-12-01
影响因子：
2.9
通讯作者：
Burnham, Carey-Ann D.
中科院分区：
文献类型：
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作者：
El Feghaly, Rana E.;Stamm, Jennifer E.;Burnham, Carey-Ann D.
常用的药敏试验方法（如Kirby-Bauer青霉素纸片扩散法和头孢硝肟β-内酰胺酶检测法）可能无法可靠地检测β-内酰胺酶的产生。我们分析了105个明显的青霉素敏感金黄色葡萄球菌分离株，使用多种方法来检测β-内酰胺酶的生产。聚合酶链反应检测10株（9.5%）β-内酰胺酶基因。bla（Z）阳性和阴性菌株的平均纸片扩散带直径分别为34和38 mm（P
Beta-lactamase production may not be reliably detected by commonly used susceptibility testing methods such as Kirby-Bauer penicillin disk diffusion and nitrocefin beta-lactamase detection. We assayed 105 apparently penicillin-susceptible Staphylococcus aureus isolates using multiple methods to detect beta-lactamase production. The bla(Z) beta-lactamase gene was detected by polymerase chain reaction in 10 (9.5%) of the 105 isolates. The average disk diffusion zone diameter was 34 and 38 mm for the bla(Z)-positive and -negative isolates, respectively (P