EFFECT OF PROTEASES AND THEIR INHIBITORS ON NEURITE OUTGROWTH FROM NEONATAL MOUSE SENSORY GANGLIA IN CULTURE
EFFECT OF PROTEASES AND THEIR INHIBITORS ON NEURITE OUTGROWTH FROM NEONATAL MOUSE SENSORY GANGLIA IN CULTURE
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DOI：
10.1016/0006-8993(86)91250-3
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发表时间：
1986-11-19
期刊：
影响因子：
2.9
通讯作者：
SEEDS, NW
中科院分区：
文献类型：
--
作者：
HAWKINS, RL;SEEDS, NW
发育中的神经元和许旺细胞已被证明分泌蛋白酶。通过加入特定的蛋白酶抑制剂，研究了这些蛋白酶对培养的感觉神经节神经突生长的影响。将新生小鼠背根神经节直接培养于塑料培养皿上，在无血清N2培养基中加入不同的蛋白酶抑制剂。大豆胰蛋白酶抑制剂被发现在体外4天神经突起生长的程度增加一倍。卵类粘蛋白胰蛋白酶抑制剂和亮抑酶肽也增加神经突生长，而α 1-抗胰蛋白酶、抗痛剂和苯甲基磺酰氟引起较小的作用。此外，添加胰蛋白酶或凝血酶抑制神经突起的生长，抑制可以逆转大豆胰蛋白酶抑制剂，而外源性纤溶酶原或尿激酶只有在高浓度的抑制。因此，神经突生长可能需要一个密切调节的蛋白酶分泌和蛋白酶抑制剂的生产系统。
Developing neurons and Schwann cells have been shown to secrete proteases. The influence of these proteases on neurite outgrowth by cultured sensory ganglia was examined by adding specific protease inhibitors. Neonatal mouse dorsal root ganglia were cultured directly on tissue-culture plastic dishes in serum-free N2 medium with different protease inhibitors. Soybean trypsin inhibitor was found to double the extent of neurite outgrowth by 4 days in vitro. Ovomucoid trypsin inhibitor and leupeptin also increased neurite outgrowth, while .alpha.1-antitrysin, antipain and phenylmethylsulfonyl fluoride elicited a smaller effect. Furthermore, added trypsin or thrombin inhibited neurite outgrowth and the inhibition could be reversed by soybean trypsin inhibitor, while exogenous plasminogen or urokinase were inhibitory only at high concentrations. Thus neurite outgrowth probably requires a closely regulated system of protease secretion and protease inhibitor production.