An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water
An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water
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DOI：
10.1016/j.mimet.2005.04.027
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发表时间：
2005-09-01
影响因子：
2.2
通讯作者：
Fintschenko, Y
中科院分区：
文献类型：
--
作者：
Lapizco-Encinas, BH;Davalos, RV;Fintschenko, Y
介电电泳（DEP）是由外加电场梯度引起的粒子运动，可以非破坏性地浓缩微生物。在基于绝缘体的介电电泳（iDEP）中，绝缘微结构产生非均匀电场以驱动微系统中的介电电泳。本文描述了iDEP设备在直流电场和压力梯度下操作时去除和浓缩细菌细胞、孢子和病毒的性能。当介电电泳克服电致动或平流时，这种装置可以选择性地捕获颗粒。在玻璃蚀刻的DEP装置中，在宽范围的直流外加电场下观察到标记微生物的介电泳捕获行为。当施加高于粒子特定阈值的场时，粒子被可逆地捕获在装置中。枯草芽孢杆菌孢子和烟草花叶病毒（TMV）的实验表现出更高的诱捕阈值比细菌细胞。iDEP装置的特征在于浓缩因子和去除效率。在本研究所用的实验条件下，初始稀释度为1 × 105个细胞/ml，大肠杆菌细胞的浓缩因子为3000 ×，去除效率接近100%。这些结果是用于浓缩和去除水中微生物的DEP装置的第一个表征。(c)2005 Elsevier B. V.保留所有权利。
Dielectrophoresis (DEP), the motion of a particle caused by an applied electric field gradient, can concentrate microorganisms non-destructively. In insulator-based dielectrophoresis (iDEP) insulating microstructurres produce non-uniform electric fields to drive DEP in microsystems. This article describes the performance of an iDEP device in removing and concentrating bacterial cells, spores and viruses while operated with a DC applied electric field and pressure gradient. Such a device can selectively trap particles when dielectrophoresis overcomes electrokinesis or advection. The dielectrophoretic trapping behavior of labeled microorganisms in a glass-etched DEP device was observed over a wide range of DC applied electric fields. When fields higher than a particle-specific threshold are applied, particles are reversibly trapped in the device. Experiments with Bacillus subtilis spores and the Tobacco Mosaic Virus (TMV) exhibited higher trapping thresholds than those of bacterial cells. The iDEP device was characterized in terms of concentration factor and removal efficiency. Under the experimental conditions used in this study with an initial dilution of I X 105 cells/ml, concentration factors of the order of 3000x and removal efficiencies approaching 100% were observed with Escherichia coli cells. These results are the first characterization of an DEP device for the concentration and removal of microbes in water. (c) 2005 Elsevier B.V. All rights reserved.