CONTROL OF YEAST GAL GENES BY MIG1 REPRESSOR - A TRANSCRIPTIONAL CASCADE IN THE GLUCOSE RESPONSE
CONTROL OF YEAST GAL GENES BY MIG1 REPRESSOR - A TRANSCRIPTIONAL CASCADE IN THE GLUCOSE RESPONSE
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DOI：
10.1002/j.1460-2075.1991.tb04901.x
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发表时间：
1991-11-01
期刊：
影响因子：
11.4
通讯作者：
RONNE, H
中科院分区：
文献类型：
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作者：
NEHLIN, JO;CARLBERG, M;RONNE, H
葡萄糖阻遏是酵母中的一种全局调节机制。我们已经研究了葡萄糖如何调节半乳糖发酵所需的GAL基因。我们发现GAL基因受转录级联反应控制。因此，编码GAL基因激活剂的GAL4被MIG1抑制，MIG1是一种与GAL4启动子结合的锌指蛋白。MIG1在控制GAL基因中具有双重作用，因为MIG1还结合GAL 1的启动子，GAL 1是由GAL 4调节的基因。MIG1的破坏与GAL80的破坏协同相互作用，GAL80是一种参与半乳糖诱导的基因。这表明对葡萄糖的MIG1依赖性反应通过诱导途径的下调而放大。
Glucose repression is a global regulatory mechanism in yeast. We have investigated how glucose regulates the GAL genes, which are required for galactose fermentation. We found that the GAL genes are controlled by a transcriptional cascade. Thus, GAL4, which encodes an activator of the GAL genes, is repressed by MIG1, a zinc finger protein that binds to the GAL4 promoter. MIG1 has a dual role in control of the GAL genes, since MIG1 also binds to the promoter of GAL1, a gene regulated by GAL4. A disruption of MIG1 interacts synergistically with a disruption of GAL80, a gene involved in galactose induction. This suggests that the MIG1-dependent response to glucose is amplified by down-regulation of the induction pathway.