Effects of diamond-like carbon thin film and wrinkle microstructure on cell proliferation
Effects of diamond-like carbon thin film and wrinkle microstructure on cell proliferation
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DOI：
10.1016/j.diamond.2018.10.021
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发表时间：
2018-11-01
影响因子：
4.1
通讯作者：
Takeuchi, Louie
中科院分区：
文献类型：
--
作者：
Ban, Masahito;Tobe, Satoshi;Takeuchi, Louie
本文旨在阐明类金刚石碳（DLC）薄膜和通过在聚二甲基硅氧烷（PDMS）基底上沉积DLC薄膜形成的褶皱微结构对细胞增殖的影响，以期应用于细胞支架，这是控制细胞活性和行为的关键技术，用于细胞治疗和治疗应用。小鼠成肌细胞（C2 C12）的培养试验，使用基板与/不与DLC薄膜和皱纹的微观结构显示，细胞增殖大大增强了DLC薄膜的存在下，在基板上。此外，研究了沉积有DLC薄膜的PDMS基板的褶皱的尺寸如何影响细胞生长，并且发现褶皱微结构具有褶皱高度差的最佳范围，即表面粗糙度（Re）为0.7至1.0 μ m，以促进细胞增殖。
This paper aims to clarify the effects of diamond-like carbon (DLC) thin film and the wrinkle microstructure formed by depositing the DLC thin film on a poly(dimethylsiloxane) (PDMS) substrate on cell proliferation with a view to applying to cell scaffolds, which are essential technologies to control cellular activity and behaviors for cell therapy and therapeutic applications. Mouse myoblast cell (C2C12) culture tests using substrates with/without the DLC thin film and the wrinkle microstructure revealed that the cell proliferation was largely enhanced by the existence of the DLC thin film on the substrate. Moreover, it was examined how the size of the wrinkle of the DLC thin film deposited PDMS substrates affected cellular growth, and it was found that the wrinkle microstructure had the optimum range of the height difference of the wrinkle, from 0.7 to 1.0 mu m as the surface roughness (Re), for promoting cell proliferation.