Immunophenotypic quantification of M1 and M2 macrophage polarization in radicular cysts of primary and permanent teeth
Immunophenotypic quantification of M1 and M2 macrophage polarization in radicular cysts of primary and permanent teeth
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DOI：
10.1111/iej.13257
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发表时间：
2019-12-17
影响因子：
5
通讯作者：
Nelson-Filho, P.
中科院分区：
文献类型：
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作者：
Bertasso, A. S.;Leon, J. E.;Nelson-Filho, P.
目的定量分析恒牙和乳牙根尖囊肿中M1和M2巨噬细胞的数量。方法所有就读于圣保罗大学里贝劳Preto牙科学院、乳牙或恒磨牙计划拔除并符合入选标准的患者：无疼痛;有/无瘘管;由于龋齿病变导致的广泛冠状面破坏，不可能修复;牙髓坏死;放射学可见的根尖牙周炎;并且没有既往治疗。切除根性囊肿，随后进行组织病理学分析，以分类炎性浸润的类型。此外，通过免疫组织化学分析定量CD 68（M1+，M2+）和CD 163（M1-，M2+）标志物。将获得的数据提交至Mann-Whitney检验，显著性水平为5%。结果恒牙囊肿平均发病年龄38.6岁，乳牙囊肿平均发病年龄5.9岁。在组织病理学分析中，没有显着差异（P = 0.87），发现在乳牙和恒牙的根囊肿之间的慢性炎症浸润的强度。在恒牙和乳牙的病变中，尽管M1和M2巨噬细胞都被检测到，但M2巨噬细胞的患病率显著更高（P < 0.05）。乳恒牙囊肿中M1和M2巨噬细胞的表达量无显著性差异（P > 0.05）。结论乳恒牙根尖囊肿中存在M1和M2巨噬细胞，其中M2细胞数量较多。与乳牙和恒牙相关的牙根囊肿中M1和M2巨噬细胞极化的免疫表型定量相似。
Aim To quantify M1 and M2 macrophages in radicular cysts of permanent (n = 14 cases) and primary teeth (n = 15 cases). Methodology All patients who attended the School of Dentistry Ribeirao Preto, University of Sao Paulo with primary teeth or permanent molars that were scheduled for extraction and fulfilled the inclusion criteria: absence of pain; presence/absence of fistulae; extensive coronal destruction due to caries lesions without possibility of restoration; pulp necrosis; radiographically visible apical periodontitis; and no previous treatment, were selected. The radicular cysts were removed and subsequently submitted to histopathologic analysis in order to classify the type of inflammatory infiltrate. In addition, CD68 (M1+, M2+) and CD163 (M1-, M2+) markers were quantified through an immunohistochemistry analysis. The data acquired were submitted to a Mann-Whitney test, with a 5% significance level. Results The patients had a mean age of 38.6 years and 5.9 years for cysts associated with permanent and primary teeth, respectively. In the histopathological analysis, no significant difference (P = 0.87) was found between radicular cysts in primary and permanent teeth regarding the intensity of the chronic inflammatory infiltrate. A significantly greater prevalence of M2 macrophages (P < 0.05) was observed in the lesions of both permanent and primary teeth, even though both M1 and M2 macrophages were detected. No significant difference (P > 0.05) was found for M1 and M2 macrophages associated with the cysts of primary and permanent teeth. Conclusion M1 and M2 macrophages were present in radicular cysts associated with primary and permanent teeth, with a greater quantity of M2 cells. The immunophenotypic quantification of M1 and M2 macrophage polarization in radicular cysts associated with primary and permanent teeth were similar.