Induction of human osteoclast-like cells by treatment of blood monocytes with anti-fusion regulatory protein-1/CD98 monoclonal antibodies
Induction of human osteoclast-like cells by treatment of blood monocytes with anti-fusion regulatory protein-1/CD98 monoclonal antibodies
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DOI：
10.1359/jbmr.1998.13.1.44
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发表时间：
1998-01-01
影响因子：
6.2
通讯作者：
Ito, Y
中科院分区：
文献类型：
--
作者：
Higuchi, S;Tabata, N;Ito, Y
我们通过外周血单核细胞与抗融合调节蛋白-1(抗-FRP-1)单抗的融合，建立了一种新的、简单的人破骨细胞形成系统。用抗FRP-1/CD98单抗培养人血单核细胞，约15h开始出现多核细胞，并随着时间的延长而增大，直到3-4d。这些融合细胞的抗酒石酸酸性磷酸酶染色呈阳性，具有大量的降钙素受体，并具有骨吸收能力，这些结果强烈表明抗FRP-1抗体诱导的多核细胞是破骨细胞。此外，在从人骨分离的破骨细胞和从人骨巨细胞瘤获得的破骨细胞样细胞中检测到FRP-1抗原。
We have developed a nem and simple system of human osteoclast formation by fusing peripheral blood monocytes with anti-Fusion Regulatory Protein-1 (anti-FRP-1) monoclonal antibody (mAb). When human blood monocytes were cultured in the presence of anti-FRP-1/CD98 mAbs, polykaryocytes began to appear at approximately 15 h and increased in size with time until 3-4 days of incubation with anti-FRP-1 mAb. These fused cells showed positive staining in tartrate-resistant acid phosphatase, possessed numerous calcitonin receptors, and were capable of bone resorption, These results strongly suggest that anti-FRP-1 antibody-induced multinucleated cells are osteoclasts. Furthermore, FRP-1 antigens were detected in osteoclasts isolated from human bone and in the osteoclast-like cells obtained from human giant cell tumors of bone.