Immunolocalization of sclerostin synthesized by osteocytes in relation to bone remodeling in the interradicular septa of ovariectomized rats.
Immunolocalization of sclerostin synthesized by osteocytes in relation to bone remodeling in the interradicular septa of ovariectomized rats.
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骨细胞合成的硬化蛋白的免疫定位与卵巢切除大鼠根间间隔骨重塑的关系。
DOI：
10.1093/jmicro/dfs052
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发表时间：
2012
期刊：
影响因子：
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et al
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文献类型：
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作者：
Guo Y.;Hasegawa T.;Amizuka N.;et al
本研究旨在阐明雌激素缺乏是否会影响牙槽骨内侧区域骨细胞衍生因子——硬化蛋白的合成。8只9周龄Wistar雌性大鼠卵巢切除（OVX）， 8只假手术（Sham）。4周后，石蜡包埋下颌第一磨牙根间间隔，进行组织化学检查。假骨中，巩膜蛋白阳性的骨细胞分布在浅层，而OVX骨中，巩膜蛋白阳性的骨细胞较少。雌激素受体α和末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记阳性细胞在各组间的分布无显著差异。假性中膈区有许多破骨细胞，OVX标本显示大量破骨细胞与核因子kB配体受体激活剂的强烈免疫标记有关。与Sham标本的观察结果相反，OVX内侧区域可见复杂的水泥线网，并伴有不规则分布的骨细胞腔隙-小管系统。综上所述，雌激素缺乏似乎抑制神经根间隔内侧区域骨细胞来源的硬化蛋白合成，这一过程似乎是通过加速骨重塑介导的，而不是通过对骨细胞的直接影响。
This study aimed at elucidating whether estrogen deficiency would affect the synthesis of an osteocyte-derived factor, sclerostin, in the mesial region of alveolar bone. Eight 9-week-old Wistar female rats were ovariectomized (OVX) and eight other rats were Sham-operated (Sham). After 4 weeks, the interradicular septa of mandibular first molar were embedded in paraffin and then histochemically examined. Sclerostin-positive osteocytes were located in the superficial layer of the mesial region of Sham bones, whereas the OVX mesial region showed less sclerostin-reactive osteocytes. There was no significant difference in the distribution of estrogen receptor α and terminal deoxynucleotidyl transferase-mediated deoxyuridinetriphosphate nick end-labeling -positive cells in the groups studied. The Sham mesial region featured many osteoclasts, and OVX specimens showed numerous osteoclasts in association with intense immunolabeling of the receptor activator of the nuclear factor kB ligand. Contrary to the observations in Sham specimens, a complex meshwork of cement lines was seen in the OVX mesial region, accompanied by an irregularly distributed osteocytic lacunar–canalicular system. In conclusion, estrogen deficiency appears to inhibit osteocyte-derived sclerostin synthesis in the mesial region of the interradicular septum, in a process that seems to be mediated by accelerated bone remodeling rather than by direct effects on osteocytes.