DETECTION OF EPSTEIN-BARR VIRAL GENOMES IN REED-STERNBERG CELLS OF HODGKINS-DISEASE
DETECTION OF EPSTEIN-BARR VIRAL GENOMES IN REED-STERNBERG CELLS OF HODGKINS-DISEASE
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DOI：
10.1056/nejm198902233200806
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发表时间：
1989-02-23
影响因子：
158.5
通讯作者：
SKLAR, J
中科院分区：
文献类型：
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作者：
WEISS, LM;MOVAHED, LA;SKLAR, J
应用狭缝杂交、Southern杂交和原位杂交技术，对霍奇金S病患者活检组织中EB病毒基因组进行了检测。对16例患者的组织标本进行狭缝印迹杂交，其中3例(19%)活检组织中含有EBV DNA。用位于EBV基因组末端的含有500个碱基的串联重复序列的DNA探针进行Southern杂交，证实了3个阳性组织标本的狭缝杂交结果，并表明这些组织中EBV感染细胞是单克隆性的。对三个阳性标本和两个先前研究中的两个标本进行了原位杂交，将EBV核酸定位于所有标本中的Reed-Sternberg细胞及其变异体，其中两个标本与Reed-Sternberg细胞进行了强烈杂交，两个标本与Reed-Sternberg细胞进行了较弱但一致的杂交，一个标本与Reed-Sternberg细胞进行了焦点杂交。我们的结论是，在霍奇金S病患者的Reed-Sternberg细胞和变异体中存在EB病毒基因组，感染的细胞是单克隆性的。
We used slot blot hybridization, Southern blot hybridization, and in situ hybridization to investigate the presence of Epstein-Barr virus (EBV) genomes in biopsy tissues from patients with Hodgkin''s disease. Slot blot hybridization performed on DNA of tissue specimens from 16 patients revealed that biopsy tissue from 3 (19 percent) contained EBV DNA. Southern blot hybridization with a DNA probe containing the 500-base-pair tandem repeated sequences located at the termini of the EBV genome confirmed the findings of the slot blot hybrdization in the three positive tissue specimens and indicated the monoclonality of the EBV-infected cells in such tissues. In situ hybridization performed on the three positive specimens and on two from a previous study localized EBV nucleic acid to the Reed-Sternberg cells and variants in all specimens, with intense hybridization to Reed-Sternberg cells in two, less intense but consistent hybridization to Reed-Sternberg cells in two, and focal hybridization to Reed-Sternberg cells in one. We conclude that EBV genomes are present within Reed-Sternberg cells and variants in some patients with Hodgkin''s disease and that the infected cells are monoclonal.