Dermatopathological studies on skin lesions of MRL mice
Dermatopathological studies on skin lesions of MRL mice
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MRL小鼠皮损的皮肤病理学研究
DOI：
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发表时间：
2004
影响因子：
3
通讯作者：
Y. Hamashima
中科院分区：
文献类型：
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作者：
F. Furukawa;Hitoshi Tanaka;Kenichi Sekita;T. Nakamura;Yuji Horiguchi;Y. Hamashima
摘要 MRL-lpr/lpr(MRL/l)小鼠是一种新的人类系统性红斑狼疮(SLE)动物模型，脱毛、结痂的皮肤病变是该小鼠的特征性表现之一。我们研究了MRL/l小鼠皮肤病变的组织病理学并研究了相关的自身免疫现象。光镜观察显示5月龄时真皮内出现角化过度、棘层肥厚、颗粒增多、液化、血管舒张以及T细胞浸润真皮。免疫组织学研究表明真皮-表皮交界处 (DEJ) 存在免疫球蛋白和/或补体沉积。在一些小鼠中，2 个月时在 DEJ 处出现免疫球蛋白沉积，而 90%–100% 的 MRL/l 小鼠在 5 个月以上时出现免疫球蛋白沉积。皮肤免疫球蛋白沉积、抗DNA抗体的发生和蛋白尿之间存在时间关系。这些发现表明，MRL/l小鼠可能为研究人类SLE皮肤病变发展的生物学机制提供新的帮助。
SummaryThe MRL-lpr/lpr(MRL/l) mouse is a new animal model for human systemic lupus erythematosus (SLE) and skin lesions with hair loss and scab formation are one of the characteristic manifestations in this mouse. We investigated the histopathology of the skin lesions in MRL/l mice and studied the related autoimmune phenomenon. Light microscopical observations revealed hyperkeratosis, acanthosis, hypergranulosis, liquefaction, vasodilation in the dermis and T-cell infiltration into the dermis at the age of 5 months (mo). Immunohistological studies showed the presence of immunoglobulins and/or complement depositions at the dermal-epidermal junction (DEJ). In some mice there was deposition of immunoglobulin at the DEJ at 2 mo and in 90%–100% of MRL/l mice at over 5 mo. Temporal relationship was present among cutaneous immunoglobulin depositions, the occurrence of anti-DNA antibodies and proteinuria. These findings suggest that MRL/l mice might provide a new aid for studying the biological mechanisms of the development of skin lesions in human SLE.