Discovery of naturally processed and HLA-presented class I peptides from vaccinia virus infection using mass spectrometry for vaccine development
Discovery of naturally processed and HLA-presented class I peptides from vaccinia virus infection using mass spectrometry for vaccine development
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DOI：
10.1016/j.vaccine.2009.09.126
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发表时间：
2009-12-10
期刊：
影响因子：
5.5
通讯作者：
Poland, Gregory A.
中科院分区：
文献类型：
--
作者：
Johnson, Kenneth L.;Ovsyannikova, Inna G.;Poland, Gregory A.
开发一种更安全的天花疫苗的一个重要方法是鉴定自然处理的免疫原性牛痘衍生多肽而不是活的整个牛痘病毒。我们使用二维液相色谱-质谱仪从感染牛痘病毒(Dryvax)后的B细胞系(人类白细胞抗原IA*0201、B*1501和C*03纯合)中鉴定了116个牛痘多肽，由61个开放阅读框编码。重要的是，其中68个多肽保守在天花中，提供了对天花诱导保护的多肽的洞察力。在这68个保守的多肽中，有21个是I I氨基酸或更长的，超出了大多数预测算法的范围因此，直接鉴定自然处理和呈现的FILA多肽提供了当前用于识别潜在牛痘病毒表位的计算方法所不提供的重要信息(C)2009爱思唯尔有限公司保留所有权利。
An important approach for developing a safer smallpox vaccine is to identify naturally processed immunogenic vaccinia-derived peptides rather than live whole vaccinia virus We used two-dimensional liquid chromatography Coupled to mass spectrometry to identify 116 vaccinia peptides, encoded by 61 open reading frames, from a B-cell line (homozygous for HLA class IA*0201, B*1501, and C*03) after infection with vaccinia virus (Dryvax) Importantly, 68 of these peptides are conserved in variola, providing insight into the peptides that induce protection against smallpox. Twenty-one of these 68 conserved peptides were I I amino acids long or longer, outside of the range of most predictive algorithms Thus, direct identification of naturally processed and presented FILA peptides gives important information not provided by current Computational methods for identifying potential vaccinia epitopes (C) 2009 Elsevier Ltd All rights reserved.