A stripe rust fungal effector PstSIE1 targets TaSGT1 to facilitate pathogen infection
A stripe rust fungal effector PstSIE1 targets TaSGT1 to facilitate pathogen infection
复制标题
DOI：
10.1111/tpj.16019
复制
发表时间：
2022-11-24
期刊：
影响因子：
7.2
通讯作者：
Guo，Jun
中科院分区：
文献类型：
--
作者：
Wang，Yunqian;Liu，Cong;Guo，Jun
条锈菌（Pucciniastriiformisf. sp. triangulae，Pst）是小麦条锈病的主要病原菌，在侵染过程中可释放数百种效应子来调控寄主体内的生理过程。SGT 1（skp 1的G2等位基因的抑制因子）、RAR 1（Mla 12抗性所需）和HSP 90（热休克蛋白90）蛋白形成了一种伴侣蛋白复合物，在植物免疫中起核心调节剂的作用。然而，很少有人知道Psteffectors如何靶向这种免疫成分，以抑制植物免疫。在这里，我们确定了aPsteffector PstSIE 1与TaSGT 1在小麦中相互作用，并在早期感染阶段上调。瞬时表达PstSIE 1抑制VmE 02和PcNLP 2诱导的烟草细胞死亡PstSIE 1-RNAi构建体在小麦中的转基因表达显著降低了Pst的毒力。PstSIE 1基因在小麦中的过表达增加了锈菌脓疱的数量，减少了活性氧的积累，表明PstSIE 1基因是Pst的一个重要致病因子。发现PstSIE 1与TaRAR 1竞争结合TaSGT 1，从而破坏TaRAR 1-TaSGT 1亚复合物的形成。综上所述，PstSIE 1是一个重要的Psteffector靶向免疫组分TaSGT 1，并参与抑制小麦防御。
Puccinia striiformisf. sp.tritici(Pst), the causal agent of stripe rust, is a destructive pathogen ofTriticum aestivum(wheat), threatening wheat production worldwide.Pstdelivers hundreds of effectors to manipulate processes in its hosts during infection. The SGT1 (suppressor of the G2 allele ofskp1), RAR1 (required forMla12resistance) and HSP90 (heat‐shock protein 90) proteins form a chaperone complex that acts as a core modulator in plant immunity. However, little is known about howPsteffectors target this immune component to suppress plant immunity. Here, we identified aPsteffector PstSIE1 that interacts with TaSGT1 in wheat and is upregulated during the early infection stage. Transient expression ofPstSIE1suppressed cell death inNicotiana benthamianainduced by VmE02 and PcNLP2. Transgenic expression ofPstSIE1‐RNAi constructs in wheat significantly reduced the virulence ofPst. Overexpression ofPstSIE1in wheat increased the number of rust pustules and reduced the accumulation of reactive oxygen species (ROS), indicating thatPstSIE1functions as an important pathogenicity factor inPst. PstSIE1 was found to compete with TaRAR1 to bind TaSGT1, thus disrupting the formation of the TaRAR1–TaSGT1 subcomplex. Taken together, PstSIE1 is an importantPsteffector targeting the immune component TaSGT1 and involved in suppressing wheat defense.