A technique to visualize wound bed microcirculation and the acute effect of negative pressure
A technique to visualize wound bed microcirculation and the acute effect of negative pressure
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DOI：
10.1111/j.1524-475x.2008.00390.x
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发表时间：
2008-05-01
影响因子：
2.9
通讯作者：
Nakatsuka, Takashi
中科院分区：
文献类型：
--
作者：
Ichioka, Shigeru;Watanabe, Hiromi;Nakatsuka, Takashi
虽然以前的研究已经证明，真空辅助闭合治疗中的亚大气压增加了伤口边缘的血流量，但还没有关于伤口床血流量的数据。这项研究考察了一种可视化伤口床微循环的技术，并估计了负压的急性影响。小鼠臀部皮肤的浅层被显微手术切除，保留了真皮下血管丛。保存的血管用活体显微镜-视频-计算机系统显示为创面床微循环。创面施加3种负压(-125 mm Hg[n=12]、-500 mm Hg[n=12]、0 mm Hg[n=8])。我们的实验模型成功地定量可视化了负压作用下的伤口床微循环。负压-125 mm Hg组创面血流量在加压后即刻显著增加，卸压后持续1min，而-500 mm Hg组随时间延长血流量下降，5min后达到统计学意义水平。这些发现强化了这样一种假设，即伤口床和伤口边缘的血流增强可能有助于一定程度的负压治疗对伤口灌流的有益影响。
Although previous studies have proved that subatmospheric pressure in vacuum assisted closure therapy increased blood flow at the wound edge, no reports have presented data on blood flow in the wound bed. This study examined a technique that visualized microcirculation of the wound bed and estimated the acute effect of negative pressure. The superficial stratum of the mouse gluteal skin was microsurgically excised preserving the subdermal vascular plexus. The preserved vessels were visualized as the wound bed microcirculation using an intravital microscope-video-computer system. Three levels of negative pressure (-125 mmHg [n=12], -500 mmHg [n=12], 0 mmHg [n=8]) were applied to the wound. Our experimental model successfully and quantitatively visualized wound bed microcirculation under negative pressure application. A negative pressure of -125 mmHg significantly increased wound bed blood flow immediately after pressure application, maintained for 1 minute after pressure release, whereas in the -500 mmHg group blood flow decreased with time and reached a statistically significant level 5 minutes after pressure application. These findings reinforce the hypothesis that enhanced blood flow in the wound bed as well as at the wound edge may contribute to the beneficial effects of certain levels of negative pressure therapy for wound perfusion.