The Evolution of the Protein Corona around Nanoparticles: A Test Study
The Evolution of the Protein Corona around Nanoparticles: A Test Study
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DOI：
10.1021/nn202458g
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发表时间：
2011-09-01
期刊：
影响因子：
17.1
通讯作者：
Dawson, Kenneth
中科院分区：
文献类型：
--
作者：
Lundqvist, Martin;Stigler, Johannes;Dawson, Kenneth
在进入生物流体时，在纳米颗粒周围形成的蛋白质冠的重要性最近得到了强调。当这种日冕存在足够长的时间时，人们认为它决定了粒子的生物命运。然而，即使是这种长期存在的“硬”日冕也会随着粒子从一种生物流体转移到另一种生物流体而进化和重新平衡，这可能是长期命运的一个重要特征。在这里，我们展示了纳米颗粒从一种生物流体（等离子体）转移到另一种生物流体（细胞质液）时蛋白质冠的进化，这是纳米颗粒进入细胞的一个简单的说明性模型。虽然不能直接比较例如在摄取途径中会发生什么，但结果证实，日冕的重大进化发生在第二种生物溶液中，但最终的日冕包含其历史的“指纹”。这可以用来绘制纳米颗粒利用的运输途径，并最终预测纳米颗粒的命运和行为。
dThe importance of the protein corona formed around nanoparticles upon entering a biological fluid has recently been highlighted. This corona is, when sufficiently long-lived, thought to govern the particles' biological fate. However, even this long-lived "hard" corona evolves and re-equilibrates as particles pass from one biological fluid to another, and may be an important feature for long-term fate. Here we show the evolution of the protein corona as a result of transfer of nanopaiticles from one biological fluid (plasma) into another (cytosolic fluid), a simple illustrative model for the uptake of nanoparticles into cells. While no direct comparison can be made to what would happen in, for example, the uptake pathway, the results confirm that significant evolution of the corona occurs in the second biological solution, but that the final corona contains a "fingerprint" of its history. This could be evolved to map the transport pathways utilized by nanoparticles, and eventually to predict nanoparticle fate and behavior.