Cross-Reactivity of Fusarium spp. in the Aspergillus Galactomannan Enzyme-Linked Immunosorbent Assay
Cross-Reactivity of Fusarium spp. in the Aspergillus Galactomannan Enzyme-Linked Immunosorbent Assay
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DOI：
10.1128/jcm.05946-11
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发表时间：
2012-03-01
影响因子：
9.4
通讯作者：
Lo Cascio, Giuliana
中科院分区：
文献类型：
--
作者：
Tortorano, Anna Maria;Esposto, Maria Carmela;Lo Cascio, Giuliana
11例播散性/深层镰刀菌感染的血液病患者中有9例至少检测了两次曲霉半乳甘露聚糖（GM），在培养物中没有曲霉菌分离的情况下，重复检测结果为阳性。用GM酶联免疫吸附试验（EIA）检测了12个镰刀菌分离物的离心上清液。所有分离株在未稀释时均产生阳性反应。这些结果表明镰刀菌属的交叉反应性。与曲霉属GM一起使用，这可能构成关于Platelia EIA的特异性的缺点。
Nine of 11 hematological patients with disseminated/deep-seated Fusarium infection tested at least twice for Aspergillus galactomannan (GM) had repeated positive results in the absence of Aspergillus isolation in culture. The centrifuged supernatants of 12 Fusarium isolates were tested by a GM enzyme-linked immunosorbent assay (EIA). All the isolates produced positive reactions when tested undiluted. These results show cross-reactivity of Fusarium spp. with Aspergillus GM that may constitute a drawback with respect to the specificity of the Platelia EIA.