Chlorine dioxide-loaded poly(acrylic acid) gels for prolonged antimicrobial effect
Chlorine dioxide-loaded poly(acrylic acid) gels for prolonged antimicrobial effect
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DOI：
10.1016/j.msec.2019.01.043
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发表时间：
2019-05-01
影响因子：
7.9
通讯作者：
Zelko, Romana
中科院分区：
文献类型：
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作者：
Palcso, Barnabas;Moldovan, Zsofia;Zelko, Romana
二氧化氯被称为“理想的杀菌剂”，因其快速、安全的抗菌特性，可以成功地用作防腐剂。其局部或口服使用的显着限制之一是，由于气体的挥发性，二氧化氯在水溶液中的停留时间非常短。因此，本研究的主要目的是通过创建一个能够长时间加载气体并在作用部位逐渐释放气体的系统来增加二氧化氯作用的持续时间。为了实现这一目标，配制了各种二氧化氯和聚合物浓度的聚丙烯酸凝胶。该配方采用二因素、三层面心中心复合材料设计。凝胶的微观结构通过正电子湮没寿命光谱进行跟踪，该光谱基于正正电子素（o-Ps）寿命分布和余氯浓度，通过碘量滴定及其抗菌作用测定。结果表明该聚合物具有两种功能。一方面，由于扩散屏障抑制了气态二氧化氯的逸度，但另一方面，聚合物链与水合形式的二氧化氯形成排列的超分子结构，从而导致其持续的逸度。后者显示出最佳的聚合物浓度在研究范围内（0.1-0.3% w/w）的函数。 o-Ps 寿命分布证实了配方的微观结构变化，并且与分析和微生物学评估非常一致。负载二氧化氯的生物粘附凝胶的应用可能是有效和安全治疗局部感染的有前途的替代方案。
Chlorine dioxide, the so-called "ideal biocide", can be successfully applied as an antiseptic agent based on its rapid and safe antimicrobial property. One of the significant limitations of its topical or oral use is that the chlorine dioxide residence time in aqueous solution is very short due to the volatility of the gas. Therefore, the primary purpose of the present study was to increase the duration of chlorine dioxide effect by creating a system capable of loading the gas for a prolonged time and gradually releasing it at the site of action. Poly(acrylic acid) gels of various chlorine dioxide and polymer concentrations were formulated to achieve this goal. A two-factor, three-level face-centred central composite design was applied for the formulation. The microstructure of the gels was tracked by positron annihilation lifetime spectroscopy based on the ortho-positronium (o-Ps) lifetime distributions with the residual chlorine concentrations, determined by iodometric titration and their antibacterial effects. The results indicate that the polymer possesses two functions. On the one hand, as a diffusion barrier inhibits the fugacity of the gaseous chlorine dioxide but on the other side, the polymer chains form an arranged supramolecular structure with the hydrated forms of chlorine dioxide thus resulting in its sustained fugacity. The latter showed optimum as a function of the polymer concentration in the investigated range (0.1-0.3% w/w). The o-Ps lifetime distributions confirmed the microstructural changes of the formulations and were in good agreement with the analytical and microbiological evaluation. The application of chlorine dioxide-loaded bioadhesive gels could be a promising alternative for the effective and safe treatment of topical infections.