Oct4 is required for primordial germ cell survival
Oct4 is required for primordial germ cell survival
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DOI：
10.1038/sj.embor.7400279
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发表时间：
2004-11-01
期刊：
影响因子：
7.7
通讯作者：
Tomilin, A
中科院分区：
文献类型：
--
作者：
Kehler, J;Tolkunova, E;Tomilin, A
以往的研究表明，Oct4在维持内细胞团细胞(ICM)和胚胎干细胞的多能性方面具有重要作用。然而，Oct4基因缺失的纯合子胚胎在植入时死亡，因此无法进一步分析发育过程中的基因功能。我们使用条件性Cre/loxP基因打靶策略来评估Oct4在原始生殖细胞(PGCs)中的功能。Oct4功能的丧失会导致PGCs的凋亡，而不是像Oct4缺陷的ICM细胞那样分化为滋养外胚层。这些新的结果表明，Oct4在维持哺乳动物生殖系的活力方面具有以前未知的功能。
Previous studies have shown that Oct4 has an essential role in maintaining pluripotency of cells of the inner cell mass (ICM) and embryonic stem cells. However, Oct4 null homozygous embryos die around the time of implantation, thus precluding further analysis of gene function during development. We have used the conditional Cre/loxP gene targeting strategy to assess Oct4 function in primordial germ cells (PGCs). Loss of Oct4 function leads to apoptosis of PGCs rather than to differentiation into a trophectodermal lineage, as has been described for Oct4-deficient ICM cells. These new results suggest a previously unknown function of Oct4 in maintaining viability of mammalian germline.