Autoimmune-related pancreatitis is associated with autoantibodies and a Th1/Th2-type cellular immune response
Autoimmune-related pancreatitis is associated with autoantibodies and a Th1/Th2-type cellular immune response
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DOI：
10.1016/s0016-5085(00)70264-2
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发表时间：
2000-03-01
期刊：
影响因子：
29.4
通讯作者：
Chiba, T
中科院分区：
文献类型：
--
作者：
Okazaki, K;Uchida, K;Chiba, T
背景与目的：虽然自身免疫可能参与了部分胰腺炎的发生，但其机制尚不清楚。为了阐明这一点，我们研究了自身免疫相关性胰腺炎（AIP）患者的血清自身抗体、淋巴细胞亚群和细胞免疫应答的Th 1/Th 2平衡。方法：研究了17例AIP患者（8例男性和9例女性;年龄，53.2 ± 13.0岁）。采用酶联免疫吸附试验（ELISA）或间接荧光抗体法检测自身抗体，包括抗乳铁蛋白（ALF）或碳酸酐酶II抗体（ACA-II）。用流式细胞术和ELISA法检测细胞内细胞因子（IFN γ和IL 4）和外周血淋巴细胞亚型。结果：17例患者均检测到一种以上的自身抗体。17例患者中13例检测到抗核抗体，13例检测到ALF抗体，10例检测到ACA-Ⅱ抗体，5例检测到类风湿因子，3例检测到抗平滑肌抗体，但均未检测到抗线粒体抗体。血清ACA-II与LF抗体水平不相关。外周血中HLA-DR（+）CD 8（+）和HLA-4-DR（+）CD 4（+）细胞明显增多（P < 0.05）。CD 4+细胞产生干扰素γ及分泌水平显著高于对照组（P < 0.05），而白细胞介素4无明显升高。结论：AIP的发生可能与抗CA-Ⅱ或LF的自身免疫机制及Th 1型免疫应答有关。
Background & Aims: Although autoimmunity may be involved in some cases of pancreatitis, the mechanism is still unknown. To clarify this, we studied serum autoantibodies, subsets of lymphocytes, and the Th1/ Th2 balance of cellular immune responses in patients with autoimmune-related pancreatitis (AIP), Methods: Seventeen patients with AIP (8 men and 9 women; age, 53.2 +/- 13.0 years) were studied. Autoantibodies including antilactoferrin (ALF) or carbonic anhydrase II antibody (ACA-II) were examined using the enzyme-linked immunosorbent assay (ELISA) or the indirect fluorescein antibody method. Intracellular cytokines (interferon gamma and interleukin 4) and subtypes of peripheral blood lymphocytes were examined by flow cytometry and ELISA, Results: More than one autoantibody was observed in all 17 patients. Serum antinuclear antibody was detected in 13 of 17 patients, ALF antibody in 13, ACA-II antibody in 10, rheumatoid factor in 5; and anti-smooth muscle antibody in 3, but antimitochondrial antibody in none. The serum levels of ACA-II and LF antibody were not correlated. HLA-DR(+)CD8(+) and HL4-DR(+)CD4(+) cells were significantly increased in peripheral blood (P < 0.05). CD4(+) cells producing interferon gamma and the secreted levels were significantly increased compared with those in controls (P < 0.05), but interleukin 4 was not increased. Conclusions: An autoimmune mechanism against CA-II or LF, and Th1-type immune response, may be involved in AIP.