Chemical profile of the active fraction of Yi-Gan San by HPLC-DAD-Q-TOF-MS and its neuroprotective effect against glutamate-induced cytotoxicity
Chemical profile of the active fraction of Yi-Gan San by HPLC-DAD-Q-TOF-MS and its neuroprotective effect against glutamate-induced cytotoxicity
复制标题
通过 HPLC-DAD-Q-TOF-MS 分析益肝散活性成分的化学特征及其对谷氨酸诱导的细胞毒性的神经保护作用。
DOI：
10.1016/s1875-5364(14)60130-8
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发表时间：
2014-11-01
影响因子：
4.6
通讯作者：
Feng Feng
中科院分区：
文献类型：
--
作者：
Chen Han;Shi Yuan-Yuan;Feng Feng
益肝散（YGS）是一种治疗痴呆相关症状的中药，此前曾被分馏。 YGS40 是一种活性成分，对谷氨酸诱导的细胞毒性具有神经保护作用。在本研究中，高效液相色谱法结合二极管阵列检测和四极杆飞行时间质谱法用于其化学成分的鉴定和定量研究。 YGS40 的化学特征由 64 种已鉴定或初步表征的化合物组成。与单一植物煎剂相比，混合煎剂中主要标志化合物的含量显着增加。结果表明，YGS40 中大多数成分的分析精度很高，并建立了单一煎煮过程和混合煎煮过程之间主要标记化合物的定量变化。
Yi-Gan San (YGS), a traditional Chinese medicine for dementia-related symptoms, was previously fractionated. One active fraction, YGS40 exhibited a neuroprotective effect against glutamate-induced cytotoxicity. In the present study, high-performance liquid chromatography, coupled with diode-array detection and quadrupole time-of-flight mass spectrometry, was applied for the identification of its chemical constituents and for quantification studies. The chemical profile of YGS40 consisted of sixty-four identified or tentatively characterized compounds. The levels of the major marker compounds increased significantly in the mixed decoction compared with those in the single plant decoction. The results suggest the high precision of the analyses of most of the constituents in YGS40 and establish the quantitative variations of the major marker compounds between the single and mixed decoction processes.