MICROTUBULE DISRUPTION INDUCED BY ESTRADIOL IN ESTROGEN RECEPTOR-POSITIVE AND RECEPTOR-NEGATIVE HUMAN BREAST-CANCER CELL-LINES
MICROTUBULE DISRUPTION INDUCED BY ESTRADIOL IN ESTROGEN RECEPTOR-POSITIVE AND RECEPTOR-NEGATIVE HUMAN BREAST-CANCER CELL-LINES
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DOI：
10.1093/carcin/15.9.1875
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发表时间：
1994-09-01
期刊：
影响因子：
4.7
通讯作者：
SATO, Y
中科院分区：
文献类型：
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作者：
AIZUYOKOTA, E;ICHINOSEKI, K;SATO, Y
用抗β-微管蛋白抗体的间接免疫荧光法检测雌激素对细胞质微管网络的影响。雌激素是一种自然产生的雌激素，在人乳腺癌细胞株MCF-7和MDA-MB-231中，雌二醇在间期减少了细胞质微管纤维的数量。由于MDA-MB-231是雌激素受体阴性的细胞系，雌二醇诱导的微管破坏似乎不依赖于雌二醇与受体的结合。诱导50%细胞微管断裂所需的雌二醇有效浓度(EC(50))：MCF-7细胞为81mU M，MDA-MB-231细胞为82mU M。合成雌激素己烯雌酚也可引起微管纤维减少，MCF-7细胞的EC(50)值为48 mU M，MDA-MB-231细胞的EC(50)值为50 mU M。经雌激素处理的微管解聚细胞经洗涤后，换成新鲜的培养液，3h内细胞内重新出现微管网络，加入雌二醇(50mU/L)培养4d后，细胞生长完全受抑。雌酮对微管网络和细胞增殖的影响较小。这些结果提示，雌二醇诱导的微管破坏与其抑制细胞生长密切相关。
Effects of estrogens on the cytoplasmic microtubule network were examined by the indirect immunofluorescence method using anti-beta-tubulin antibody. Estradiol, a naturally occurring estrogen, decreased the amount of cytoplasmic microtubule fibers during interphase in the human breast cancer cell lines MCF-7 and MDA-MB-231, Since MDA-MB-231 is an estrogen receptor-negative cell line, estradiol-induced microtubule disruption seems to be independent of estradiol binding to receptors. The effective concentration of estradiol required for induction of microtubule disruption in 50% of the cells (EC(50)) was 81 mu M for MCF-7 cells and 82 mu M for MDA-MB-231 cells. A synthetic estrogen, diethylstilbestrol, also induced a decrease in microtubule fibers, with an EC(50) value of 48 mu M for MCF-7 cells and 50 mu M for MDA-MB-231 cells. When estrogen-treated and microtubule-depolymerized cells were washed and the medium was replaced with fresh, intracellular microtubule networks reappeared within 3 h. When MCF-7 cells were cultured for 4 days with estradiol (50 mu M), cell growth was completely inhibited. However, estrone affected the microtubule network and cell proliferation only slightly. These results suggest that estradiol-induced microtubule disruption is closely related to its inhibitory effect on cell growth.