A dried blood sample on filter paper is suitable for detecting Plasmodium falciparum gametocytes by reverse transcription polymerase chain reaction
A dried blood sample on filter paper is suitable for detecting Plasmodium falciparum gametocytes by reverse transcription polymerase chain reaction
复制标题
DOI：
10.1016/j.actatropica.2008.05.001
复制
发表时间：
2008-08-01
期刊：
影响因子：
2.7
通讯作者：
Taniguchi, Koki
中科院分区：
文献类型：
--
作者：
Maeno, Yoshimasa;Nakazawa, Shusuke;Taniguchi, Koki
人外周血配子体检测是疟疾调查的重要手段之一。我们尝试用逆转录聚合酶链反应（RT-PCR）检测滤纸干血中的恶性疟原虫配子体。通过现场样本分析，131例恶性疟疾患者中67例检测到pfg377 mRNA 3区特异性RT-PCR产物。实时定量RT-PCR检测阳性样品的最低检出量为0.03个配子细胞/ μ l。配子细胞阳性率与性别、年龄无关。恶性疟原虫单一感染组配子体出现频率高于混合感染组（P < 0.01）。在这项研究中，131例患者中有47例无症状。47例患者中有18例出现pfg377 mRNA表达。此外，在pfg377 mrna阳性患者中检测到pfg377 3区的4个等位基因，67例pfg377 mrna阳性患者中有13例携带不止一个产生配子细胞的克隆。这些结果表明，滤纸上的干血可用于疟疾传播的分子流行病学研究和配子体靶向控制。(C) 2008 Elsevier B.V.版权所有
The detection of gametocytes in human peripheral blood is one of the most important measures in a malaria survey. We attempted to detect gametocytes of Plasmodium falciparum by reverse transcription polymerase chain reaction (RT-PCR) of dried blood on filter paper. On field samples analysis, the specific RT-PCR products for region 3 of pfg377 mRNA were observed in 67 of 131 falciparum malaria patients. The minimum detection level of RT-PCR-positive samples was 0.03 gametocytes/mu l on quantitative real-time RT-PCR. Gametocyte positive rate was not dependent on sex or age. A higher frequency of gametocytes was found in single P. falciparum infection than in mixed species infection (P < 0.01). In this study, 47 of the 131 patients were asymptomatic. Eighteen of these 47 patients showed pfg377 mRNA expression. Moreover, four alleles of region 3 of pfg377 were detected in pfg377 mRNA-positive patients and 13 of 67 pfg377 mRNA-positive patients carried more than one gametocyte-producing clone. These results suggest that dried blood on filter paper is a useful for a molecular epidemiologic study of malaria transmission and gametocyte-targeted control. (C) 2008 Elsevier B.V. All rights reserved.