Diffusion tensor imaging measures of white matter compared to myelin basic protein immunofluorescence in tissue cleared intact brains
Diffusion tensor imaging measures of white matter compared to myelin basic protein immunofluorescence in tissue cleared intact brains
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DOI：
10.1016/j.dib.2016.12.018
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发表时间：
2017-02-01
期刊：
影响因子：
1.2
通讯作者：
Malhotra, Anil K.
中科院分区：
文献类型：
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作者：
Chang, Eric H.;Argyelan, Miklos;Malhotra, Anil K.
我们提供在完整小鼠大脑上进行的离体扩散张量成像 (DTI) 和透明脂质交换、解剖学刚性、成像/免疫染色兼容的组织水凝胶 (CLARITY) 的组合数据集。将 DTI 衍生的分数各向异性 (FA)、径向扩散率 (RD) 和轴向扩散率 (AD) 测量值与基于抗体的髓磷脂碱性蛋白 (MBP) 标记（通过荧光显微镜测量）进行比较。我们使用定制的 CLARITY 水凝胶溶液来促进全脑组织透明化和随后的免疫标记。我们描述了 CLARITY 如何与磁共振成像兼容，旨在促进未来可能将无创成像与 3D 免疫组织化学相结合的多模态成像研究。这些数据和方法与随附的题为“髓鞘形成在白质完整性测量中的作用：CLARITY完整大脑的扩散张量成像和双光子显微镜相结合”的研究文章相关（E.H. Chang、M. Argyelan、M. Aggarwal、T-S. Chandon、K.H. Karlsgodt、S. Mori、A.K. Malhotra，2016）[1]。 (C) 2016 年作者。由爱思唯尔公司出版
We provide datasets from combined ex vivo diffusion tensor imaging (DTI) and Clear Lipid-exchanged, Anatomically Rigid, Imaging/ immunostaining compatible, Tissue hYdrogel (CLARITY) performed on intact mouse brains. DTI-derived measures of fractional anisotropy (FA), radial diffusivity (RD), and axial diffusivity (AD) were compared to antibody-based labeling of myelin basic protein (MBP), as measured by fluorescence microscopy. We used a customized CLARITY hydrogel solution to facilitate whole brain tissue clearing and subsequent immunolabeling. We describe how CLARITY was made compatible with magnetic resonance imaging with the intention of facilitating future multimodal imaging studies that may combine noninvasive imaging with 3D immunohistochemistry. These data and methods are related to the accompanying research article entitled, 'The role of myelination in measures of white matter integrity: Combination of diffusion tensor imaging and two-photon microscopy of CLARITY intact brains' (E.H. Chang, M. Argyelan, M. Aggarwal, T-S. Chandon, K.H. Karlsgodt, S. Mori, A.K. Malhotra, 2016) [1]. (C) 2016 The Authors. Published by Elsevier Inc.