Adaptation of the Na+/glucose cotransporter following intestinal resection.
Adaptation of the Na+/glucose cotransporter following intestinal resection.
复制标题
肠切除术后钠/葡萄糖协同转运蛋白的适应。
DOI：
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发表时间：
1994
影响因子：
2.2
通讯作者：
Stanley W. Ashley
中科院分区：
文献类型：
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作者：
Oscar J. Hines;Anton J. Bilchik;Michael J. Zinner;Michael J. Skotzko;A.James Moser;David W. McFadden;Stanley W. Ashley
大面积小肠切除后，剩余的肠道适应以补偿失去的吸收能力。虽然Na+/葡萄糖协同转运蛋白在小肠的营养、液体和电解质转运中起着关键作用，但其在切除后适应中的作用尚未确定。为了检验这一点，我们试图确定是否有Na+/葡萄糖协同转运蛋白，SGTL 1，在信使RNA水平的表达变化。刘易斯大鼠行横断或70%小肠切除再吻合术。手术后每隔一段时间处死动物。空肠近端吻合和回肠和结肠远端吻合收获和分析Na+/葡萄糖mRNA的逆转录-聚合酶链反应和Southern印迹。通过32 P标记对印迹进行半定量，并将其标准化为β-肌动蛋白。组织切片和DNA，RNA和蛋白质含量分析显示增生性变化。空肠切除后1周Na+/葡萄糖协同转运蛋白mRNA表达显著增加（P < 0.05），并持续升高。在回肠中，在6小时时几乎增加了5倍，并且在整个研究期间持续增加（P < 0.05）。再吻合远端回肠的早期反应高于空肠（P < 0.05）。相反，在结肠中检测到的少量转运蛋白mRNA没有变化。这些结果表明，除了粘膜增生，肠切除的反应涉及上调转运mRNA的个别肠上皮细胞。Na+/葡萄糖协同转运蛋白的转录增加似乎是肠道的早期反应，并且在切除后维持整体肠道转运能力方面可能很重要。
Following massive small bowel resection, the remaining intestine adapts to compensate for lost absorptive capacity. Although the Na+/glucose cotransporter plays a critical role in nutrient, fluid, and electrolyte transport in the small intestine, its role in adaptation following resection has not been defined. To examine this, we sought to determine whether there were changes in the expression of the Na+/glucose cotransporter, SGTL1, at the messenger RNA level. Lewis rats underwent either transection or 70% small bowel resection and reanastomosis. The animals were sacrificed at intervals following operation. Jejunum proximal to the anastomosis and ileum and colon distal to the anastomosis were harvested and analyzed for Na+/glucose mRNA by reverse transcriptase-polymerase chain reaction and Southern blot. Blots were semiquantitated by 32P labeling and standardized to beta-actin. Histologic sections and analysis of DNA, RNA, and protein content revealed hyperplastic changes. Following resection, mRNA for the Na+/glucose cotransporter in the jejunum increased significantly (P < 0.05) by 1 week and remained elevated. In the ileum, an almost fivefold increase occurred at 6 hr and persisted throughout the study (P < 0.05). The early response was greater in the ileum, distal to the reanastomosis, than that in the jejunum (P < 0.05). In contrast, there was no change in the small amount of transporter mRNA detected in the colon. These results suggest that, in addition to mucosal hyperplasia, the intestinal response to resection involves upregulation of transporter mRNA by the individual enterocyte. This transcriptional increase in the Na+/glucose cotransporter appears to be an early response by the intestine and may be important in maintaining overall intestinal transport capacity following resection.