Characterization and localization to chromosome 7 of psihGABPalpha, a human processed pseudogene related to the ets transcription factor, hGABPalpha.
Characterization and localization to chromosome 7 of psihGABPalpha, a human processed pseudogene related to the ets transcription factor, hGABPalpha.
复制标题
psihGABPalpha 的特征和在 7 号染色体上的定位，psihGABPalpha 是与 ets 转录因子 hGABPalpha 相关的人类加工假基因。
DOI：
10.1016/s0378-1119(99)00167-5
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发表时间：
1999
期刊：
影响因子：
3.5
通讯作者：
Rosmarin,AG
中科院分区：
文献类型：
--
作者：
Luo,M;Shang,J;Yang,Z;Simkevich,CP;Jackson,CL;King,TC;Rosmarin,AG
GABP 是一种异聚转录因子复合物，由 ets 相关蛋白 GABPα 和 Notch 相关蛋白 GABPβ 组成。我们分离了一个与人 GABPα cDNA 高度同源和共线性的人基因组 DNA 片段，但缺乏内含子。这种经过加工的假基因ψhGABPα在U937人骨髓细胞中表达为RNA，但与ATG起始蛋氨酸密码子相对应的位点的突变阻止了其翻译成蛋白质。使用体细胞杂交作图面板将该假基因定位于 7 号染色体，并且与定位于 21 号染色体的真实 GABPα 不同线。我们已经鉴定出 ψhGABPα，这是一种新的 GABPα 相关加工假基因，在人骨髓细胞中表达为 RNA 转录本。
GABP is a heteromeric transcription factor complex which consists of the ets related protein, GABPα, and the Notch-related protein, GABPβ. We isolated a human genomic DNA fragment which is highly homologous and colinear with human GABPα cDNA, but which lacks introns. This processed pseudogene, ψhGABPα, is expressed as RNA in U937 human myeloid cells, but a mutation at the site that corresponds to the ATG start methionine codon prevents its translation into protein. The pseudogene was localized to chromosome 7 using a somatic cell hybrid mapping panel and it is not syntenic with authentic GABPα, which was localized to chromosome 21. We have identified ψhGABPα, a novel, GABPα-related processed pseudogene which is expressed as a RNA transcript in human myeloid cells.