Vascularization of plastic calcium phosphate cement in vivo induced by in-situ-generated hollow channels.
Vascularization of plastic calcium phosphate cement in vivo induced by in-situ-generated hollow channels.
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DOI：
10.1016/j.msec.2016.05.106
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发表时间：
2016-11
期刊：
影响因子：
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通讯作者：
Tao Yu;Chao Dong;Zhonghua Shen;Yan Chen;Bo Yu;Haishan Shi;Changren Zhou;Jiandong Ye
中科院分区：
文献类型：
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作者：
Tao Yu;Chao Dong;Zhonghua Shen;Yan Chen;Bo Yu;Haishan Shi;Changren Zhou;Jiandong Ye
尽管磷酸钙骨水泥（CPC）是有希望的骨修复治疗，缓慢的生物降解和结构中的血管化不足的负面影响其临床应用。研究了一种自固化CPC与明胶纤维的复合物，以测试这种组织工程策略的实用性，以支持快速和广泛的血管化过程。CPC中相互连通的中空通道是明胶纤维在体内溶解后形成的。与对照相比，CPC-明胶样品表现出相对良好/增强的机械性能。当在体内植入时，材料中预先建立的血管网络与宿主血管紧密结合，并加速整个组织结构中的血管浸润。不同的通道尺寸诱导不同的体内血管化行为。结果表明，250 μm大小的通道可增加代表性血管生成因子HIF 1 α、PLGF和迁移因子CXCR 4的表达，有利于小血管的形成。另一方面，500 μm大小的通道能促进VEGF-A的表达，有利于大血管的发育。值得注意的是，在缺氧条件下，通道的交叉区域具有较高的侵袭、出芽和血管生成潜力，因为在那里观察到更多的HIF 1 α阳性细胞。支架边缘可见CD 31阳性管腔，提示血管从宿主体内通过通道向材料内长入，这得益于HIF 1 α表达的逐渐增加。该材料结合了原位自固化、可塑性、血管生成和骨传导性等优点，有望促进骨再生的有效应用。
Despite calcium phosphate cement (CPC) is promising for bone repair therapy, slow biodegradation and insufficient vascularization in constructs negatively impacts its clinical application. A self-setting CPC composited with gelatin fiber is investigated to test the utility of this tissue engineering strategy to support rapid and extensive vascularization process. The interconnected hollow channels in CPC are formed after dissolution of gelatin fibersin vivo. The CPC-gelatin samples exhibit relatively decent/enhanced mechanical property, compared to the control. When implantedin vivo, the pre-established vascular networks in material anastomose with host vessels and accelerate vascular infiltration throughout the whole tissue construct. Different channel sizes induce different vascularization behaviorsin vivo. Results indicate that the channel with the size of 250 μm increases the expression of the representative angiogenic factors HIF1α, PLGF and migration factor CXCR4, which benefit the formation of small vessels. On the other hand, the channel with the size of 500 μm enhances VEGF-A expression, which benefit the development of large vessels. Notably, the intersection area of channels has high invasive, sprouting and vasculogenesis potential under hypoxic condition, because more HIF1α-positive cells are observed there. Observation of the CD31-positive lumen in the border of scaffold indicates the ingrowth of blood vessels from its host into material through channel, benefited from gradually increased HIF1α expression. This kind of material was suggested to promote the effective application of bone regeneration through the combination ofin situself-setting, plasticity, angiogenesis, and osteoconductivity.