INACTIVATION OF MYXOVIRUSES BY OXIDIZED POLYAMINES
INACTIVATION OF MYXOVIRUSES BY OXIDIZED POLYAMINES
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DOI：
10.1099/0022-1317-11-1-1
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发表时间：
1971-01-01
影响因子：
3.8
通讯作者：
DON, S
中科院分区：
文献类型：
--
作者：
BACHRACH, U;DON, S
精胺，氧化的部分纯化血清胺氧化酶，灭活流感，纽卡斯尔病和仙台病毒。通过测定鸡胚中血凝素的产生来确定灭活率，其取决于药物浓度、温度和暴露时间。在适当条件下，观察到所有病毒完全灭活。氧化亚精胺也有抑制作用，但浓度较高。完全灭活的纽卡斯尔病病毒和仙台病毒体外凝集红细胞和融合的埃利希腹水细胞。电子显微镜显示氧化精胺对灭活病毒的完整性几乎没有影响。用硼氢化钠还原后，氧化精胺的杀病毒活性被取消，这意味着醛基在灭活过程中的重要性。
Spermine, oxidized by partially purified serum amine oxidase, inactivated influenza, Newcastle disease and Sendai viruses. The rate of inactivation, determined by assaying the production of haemagglutinins in chick embryos, depended upon the concentration of the drug, temperature, and exposure time. Under appropriate conditions a complete inactivation of all the viruses was observed. Oxidized spermidine was also inhibitory, but at higher concentrations. Completely inactivated Newcastle disease and Sendai viruses agglutinated red blood cellsin vitroand fused Ehrlich ascites cells. Electron microscopy showed that oxidized spermine had little effect on the integrity of the inactivated viruses. The virucidal activity of oxidized spermine was abolished after reduction with sodium borohydride, implying the importance of aldehyde groups in the inactivation process.