Acoustic vibration can enhance bacterial biofilm formation
Acoustic vibration can enhance bacterial biofilm formation
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DOI：
10.1016/j.jbiosc.2016.05.010
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发表时间：
2016-12-01
影响因子：
2.8
通讯作者：
Bezombes, Frederic
中科院分区：
文献类型：
--
作者：
Murphy, Mark F.;Edwards, Thomas;Bezombes, Frederic
本文探讨了低频低振幅声振动在生物膜形成中的应用。人们认为生物膜的发展受到多种环境信号的控制，并且人们投入了大量精力来研究环境因素的影响，包括：养分有效性、pH 值和温度对生物膜生长的影响。许多形成生物膜的生物体已经进化到能够在具有机械挑战性的环境中茁壮成长，例如土壤，然而，物理环境对生物膜形成的影响在很大程度上被忽视了。将铜绿假单胞菌暴露于 100、800 和 1600 Hz 的振动下 48 小时，与对照相比，导致生物膜形成显着增加，其中在 800 Hz 振动时看到最大的增长。结果还表明，生物膜形成的增加伴随着铜绿假单胞菌细胞数量的增加。还发现声振动以频率依赖的方式调节生物膜形成的空间分布。将金黄色葡萄球菌暴露于声振动也导致生物膜形成增强，在 1600 Hz 下暴露 48 小时后形成最高水平的生物膜。这些结果表明，声振动可用于控制生物膜的形成，因此提供了一种新颖且具有潜在成本效益的方法来控制一系列重要工业和医疗过程中生物膜的形成和产量。 (C) 2016 年，日本生物技术协会。版权所有。
This paper explores the use of low-frequency low-amplitude acoustic vibration on biofilm formation. Biofilm development is thought to be governed by a diverse range of environmental signals and much effort has gone into researching the effects of environmental factors including; nutrient availability, pH and temperature on the growth of biofilms. Many biofilm-forming organisms have evolved to thrive in mechanically challenging environments, for example soil yet, the effects of the physical environment on biofilm formation has been largely ignored. Exposure of Pseudomonas aeruginosa to vibration at 100, 800 and 1600 Hz for 48 h, resulted in a significant increase in biofilm formation compared with the control, with the greatest growth seen at 800 Hz vibration. The results also show that this increase in biofilm formation is accompanied with an increase in P. aeruginosa cell number. Acoustic vibration was also found to regulate the spatial distribution of biofilm formation in a frequency-dependent manner. Exposure of Staphylococcus aureus to acoustic vibration also resulted in enhanced biofilm formation with the greatest level of biofilm being formed following 48 h exposure at 1600 Hz. These results show that acoustic vibration can be used to control biofilm formation and therefore presents a novel and potentially cost effective means to manipulate the development and yield of biofilms in a range of important industrial and medical processes. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.