Exceptional paternal inheritance of plastids in Arabidopsis suggests that low-frequency leakage of plastids via pollen may be universal in plants
Exceptional paternal inheritance of plastids in Arabidopsis suggests that low-frequency leakage of plastids via pollen may be universal in plants
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DOI：
10.1111/j.1365-313x.2007.03278.x
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发表时间：
2007-12-01
期刊：
影响因子：
7.2
通讯作者：
Maliga, Pal
中科院分区：
文献类型：
--
作者：
Azhagiri, Arun K.;Maliga, Pal
拟南芥的花粉和精子细胞中不存在质体DNA。因此，在标准的遗传杂交中，质体和线粒体仅由母本遗传给种子后代。我们的目的是测试父本质体是否作为例外通过花粉传播。本杂交母本为核雄性不育株（ms1-1/ms1-1），对大霉素敏感。它被一个雄性可育的RLD-Spc1植株的花粉受精，该植株携带一个质体编码的大观霉素抗性突变。通过在父本质体DNA中编码大霉素抗性来选择具有亲本质体的幼苗。总的来说，我们的数据支持拟南芥质体的母系遗传。然而，我们报道，在拟南芥中，父本质体以较低的频率（3.9 x 10(-5)）传递给种子后代。这一观察结果将之前对Antirrhinum majus、Epilobium hirsutum、Nicotiana tabacum、矮牵牛花（Petunia hybrida）和谷类作物狗尾草（Setaria italica）的报道扩展到十字花科物种，表明在以前被认为表现出严格的母体质体遗传的植物中，通过花粉进行的低频率父本质体泄漏可能是普遍存在的。本文使用的遗传工具将有助于测试拟南芥核突变对质体遗传的影响，并允许设计突变筛选来识别控制质体遗传的核基因。
Plastid DNA is absent in pollen or sperm cells of Arabidopsis thaliana. Accordingly, plastids and mitochondria, in a standard genetic cross, are transmitted to the seed progeny by the maternal parent only. Our objective was to test whether paternal plastids are transmitted by pollen as an exception. The maternal parent in our cross was a nuclear male sterile (ms1-1/ms1-1), spectinomycin-sensitive Ler plant. It was fertilized with pollen of a male fertile RLD-Spc1 plant carrying a plastid-encoded spectinomycin resistance mutation. Seedlings with paternal plastids were selected by spectinomycin resistance encoded in the paternal plastid DNA. Our data, in general, support maternal inheritance of plastids in A. thaliana. However, we report that paternal plastids are transmitted to the seed progeny in Arabidopsis at a low (3.9 x 10(-5)) frequency. This observation extends previous reports in Antirrhinum majus, Epilobium hirsutum, Nicotiana tabacum, Petunia hybrida, and the cereal crop Setaria italica to a cruciferous species suggesting that low-frequency paternal leakage of plastids via pollen may be universal in plants previously thought to exhibit strict maternal plastid inheritance. The genetic tools employed here will facilitate testing the effect of Arabidopsis nuclear mutations on plastid inheritance and allow for the design of mutant screens to identify nuclear genes controlling plastid inheritance.